白花前胡有效成分Pd-Ia对急性肺损伤的作用及机制研究  

Protective effect of Pd-Ia on LPS-induced mouse acute lung injury and its mechanism

作　　者：周晓霞[1] 张建情 刘春晓[1] 常贺[1] 邹军[1] 薛茂强[1] 

机构地区：[1]厦门大学基础医学系,福建厦门361102

出　　处：《中国药理学通报》2016年第8期1165-1170,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81270294);福建省高校省级自然科学基金项目(No 2013J01357)

摘　　要：目的探讨白花前胡甲素(dl-praeruptorin,Pd-Ia)对脂多糖(lipopolysaccharide,LPS)诱导急性肺损伤的作用及其机制。方法气管滴注LPS建立小鼠急性肺损伤模型,PdIa腹腔注射进行干预治疗,应用组织细胞染色及定量PCR、ELISA等方法评估Pd-Ia对急性肺损伤的作用。结果病理形态学显示,Pd-Ia治疗组炎性细胞浸润明显减轻,细胞染色也显示BALF中炎性细胞数量明显减少,定量PCR及ELISA结果显示,Pd-Ia治疗组抑制了肺组织中细胞因子IL-6、TNF-α、IL-1β、趋化因子MIP-1α、MIP-2的表达。结论 Pd-Ia治疗可以明显减轻肺部的炎症反应,从而改善肺损伤。Aim To explore the protective effects of dl-praeruptorin （ Pd-Ia） against acute lung injury induced by lipopolysaccharide （ LPS ） .Methods Acute lung injury model was induced by intranasal instillation LPS, and Pd-Ia was administered by intraperitoneal in-jection after 1 h of LPS exposure .Lung tissue samples were collected after 24 h of LPS administration to in-vestigate the role of Pd-Ia in acute lung injury .Results Pathomorpholoy showed a marked improvement of in-flammatory cell infiltration in Pd-Ia treated group , cel-lular staining also indicated a marked decrease of in-flammatory cells in BALF, and quantitative PCR and ELISA revealed a significant inhibition of cytokine IL-6,TNF-α, IL-1β, and chemokine MIP-1α, MIP-2 ex-pression .Pd-Ia attenuated LPS-induced histological se-verities and TNF-α, IL-6, IL-1β,MIP-1αand MIP-2 production .Conclusion Pd-Ia can alleviate the lung injury by ameliorating inflammation in lung .

关 键 词：急性呼吸窘迫综合征 急性肺损伤 脂多糖 白花前胡甲素 炎症 细胞因子 :急性呼吸窘迫综合征 急性肺损伤 脂多糖 白花前胡甲素 炎症 细胞因子 

分 类 号：R-332[医药卫生] R284.1