B(A)02亚型分子生物学鉴定1例  

An Identification of B(A)02 Subgroup Blood

作　　者：宋艳艳[1] 周雪莹[1] 欧阳旭[1] 徐敏[1] 于笑难[1] 

机构地区：[1]辽宁省沈阳军区总医院输血科,110840

出　　处：《临床输血与检验》2016年第4期338-341,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的采用分子生物学方法进行疑难血型鉴定及其遗传发生机制分析,探究分子生物学在红细胞血型分型技术中的必要性和可行性。方法使用血清学和分子生物学方法,对1例二次献血时血型与首次记录不符且ABO正反定不符的献血者,进行血型鉴定。结果献血者血清学:与抗-A血清反应呈弱阳性(2+),与抗-B及抗-H血清反应均为强阳性(4+);与A1型红细胞呈弱阳性(2+),但与B和O型红细胞不反应;直接、间接抗球蛋白试验结果均为阴性、血清抗体筛查结果为阴性。献血者分子生物学:ABO基因第6、7外显子区存在9处变异,与A101等位基因进行比对,确定血型为B(A)02。结论分子生物学方法可以避免血清学疑难血型鉴定中的诸多影响因素,及时准确地鉴定B(A)亚型。Objective To explore the necessity and feasibility of RBC blood typing by molecular technique for unidentified blood group. Methods The blood group was identified by serological test and molecular detection. Results One case of blood donor showed weakly positive reaction to anti-A, strongly positive reaction to anti-B and anti-H, weakly positive reaction to A1 RBCs but no reaction to either B or O blood type. The sample was subjected to the detection of ABO genes and the results showed 9 mutants in Exons 6 and 7. The type B（A）02 was identified when compared with A101 allele. Conclusion Genetic detection is helpful for finding B（A） subgroup blood that can not be identified by serological test.

关 键 词：ABO亚型 B(A)血型 等位基因 血型基因分型 

分 类 号：R457.11[医药卫生—治疗学]