疑难ABO血型标本的表型与相应等位基因的检测  

Difficulty of ABO blood group specimen phenotype and gene detection

作　　者：王莹[1] 

机构地区：[1]辽宁中医药大学附属医院输血科,辽宁沈阳110032

出　　处：《中国实验诊断学》2016年第7期1113-1115,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨分析疑难ABO血型标本与其等位基因的检测,为临床遇到的疑难ABO血型标本提供可靠的血型鉴定方法。方法选取2013年8月到2014年8月期间在笔者就职医院各科室送检的临床病人血液样本9例,首先按照常规血型检测技术进行ABO血型血清学试验,确定ABO正反定型,再采用盐酸胍/蛋白酶K裂解提取法对ETDA抗凝处理后的静脉外周血进行DNA提取,再使用聚合酶链反应-序列特异性引物法(PCR-SSP)对9例血样进行ABO血型基因分型检测。结果 9例临床病人血清学表型为:AxB 2例、AB亚1例、A亚B亚1例,Ax 2例,B亚3例,9例患者基因型分别为为:A/B 4例,A/O 2例,B/O 3例,通过PCR-SSP检测所得基因型与血清学表型结果相一致。结论利用聚合酶链反应-序列特异性引物法(PCR-SSP)对血清学方法鉴定ABO血型结果为正反不定型的疑难血型标本进行基因型检测,能准确地确定患者的血型抗原,为临床疑难ABO血型患者制定安全有效的用血方案提供保障。Objective To study the analysis of difficult specimens of ABO blood group and its allele detection,for clinical encounter difficulty provide reliable blood specimens of ABO blood group identification method.Methods Selected during August 2013 to August 2014 working in the hospital each department makes clinical patient blood samples from 9cases,first of all,according to the conventional blood detection techniques for ABO serological test,determine ABO positive and negative stereotypes,again using guanidine hydrochloride/proteinase K cracking after extraction of ETDA anticoagulant treatment of peripheral vein for DNA extraction,then using the method of polymerase chain reaction sequence specific primers（PCR SSP）ABO blood group 9cases of blood sample genotyping detection.Results 9cases of clinical serological phenotype for patients：AxB in 2cases,AB,and 1case,and A and B,and 1case,Ax in 2cases,B,and 3cases,9cases of patients with genotype,respectively is：A/B 4cases,2cases of A/O,B/O 3cases,measured by PCR-SSP genotype is consistent with the results of serological phenotype.Conclusion Using the method of polymerase chain reaction sequence specific primers（PCR SSP）ABO blood group identification results of serological method for positive and negative don＇t finalize the design difficulty of blood specimens tested genotypes,to accurately determine the patient＇s blood group antigen,for clinical patients with complicated ABO blood group provide guarantee for safe and effective blood scheme.

关 键 词：疑难ABO血型标本 等位基因 聚合酶链反应-序列特异性引物法 

分 类 号：R440[医药卫生—诊断学]