细菌非编码小RNA对细菌毒力的调控作用  

Role of small noncoding RNA in the regulation of bacterial virulence

作　　者：王禄[1] 郑欣[1] 王诗达 李继遥[1] 徐欣[1] 

机构地区：[1]口腔疾病研究国家重点实验室、华西口腔医院牙体牙髓病科(四川大学) [2]口腔疾病研究国家重点实验室,华西口腔医院全科门诊(四川大学),成都610041

出　　处：《华西口腔医学杂志》2016年第4期433-438,共6页West China Journal of Stomatology

基　　金：国家自然科学基金(81200782,81371135);四川省科技厅基金(2013HH0009)~~

摘　　要：在病原菌与宿主长期的交互作用过程中，病原菌可通过调节相关基因的表达响应宿主微环境的变化，以适应宿主内环境并在宿主体内生存。过去认为，细菌的基因表达调控主要发生在转录水平。近年发现，细菌非编码小RNA（sRNA）在调控细菌致病机制方面发挥着重要作用。细菌sRNA是一类长度在50-500个核苷酸之间的非编码RNA，病原菌可感受宿主微环境的变化，通过sRNA调控自身毒力相关基因的表达，促进致病菌在宿主内的生存能力，利于致病菌对宿主的侵袭及致病。相对于各种转录因子，sRNA介导的基因表达调控可使细菌更快速、灵敏地对外界环境变化作出应答。目前已发现多种与细菌毒力及致病性相关的sRNA，可在转录后水平精细调节细菌毒力及其致病机制。本文就sRNA调控细菌毒力的分子机制及其在常见病原菌致病过程中的作用进行综述。In the long-term interaction between pathogens and host, the pathogens regulate the expression of related viru-lence genes to fit the host environment in response to the changes in the host microenvironment. Gene expression was believed to be controlled mainly at the level of transcription initiation by repressors or activators. Recent studies have revealed that small noncoding RNAs （sRNAs） are key regulators in bacterial pathogenesis. sRNA in bacteria is a noncoding RNA with length ranging from 50 to 500 nucleotides. Pathogens can sense the changes in the host environment and consequently regulate the expression of virulence genes by sRNAs. This condition promotes the ability of pathogens to survive within the host, which is beneficial to the invasion and pathogenicity of pathogens. In contrast to transcriptional factors, sRNA-mediated gene regu-lation makes rapid and sensitive responses to environmental cues. Many sRNAs involved in bacterial virulence and pathoge-nesis have been identified. These sRNAs are key components of coordinated regulation networks, playing important roles in regulating the expression of virulence genes at post-transcriptional level. This review aims to provide an overview on the mole-cular mechanisms and roles of sRNAs in the regulation of bacterial virulence.

关 键 词：非编码小RNA 转录后调控 细菌 毒力因子 

分 类 号：R780.2[医药卫生—口腔医学]