东亚飞蝗肠道内高产纤维素酶菌株筛选及鉴定  

Screening and Identification of High-Yielding Cellulase Strain from Enteric Duct in East Asian Locust (Locusta migratoria manilensis

作　　者：徐冲[1] 钟丽娟[1] 陈丽媛[1] 陈杰[1] 孙翠焕[1] 朱巍巍[1] 关艳丽[1] 

机构地区：[1]辽宁省微生物科学研究院,辽宁朝阳122000

出　　处：《微生物学杂志》2016年第3期69-72,共4页Journal of Microbiology

基　　金：国家农业科技成果转化项目(国科发农[2010]297号)

摘　　要：为微生物制剂生产筛选菌种资源。运用昆虫解剖技术取出东亚飞蝗(Locusta migratoria manilensis)肠道,采用稀释涂板法对肠道内的菌种进行分离,并利用羧甲基纤维素钠(CMC-Na)改良培养基初筛产纤维素酶菌株。结果表明,从东亚飞蝗肠道内共分离得到12株产纤维素酶菌株,均为细菌,并对纤维素酶活较高的菌株K005进行了形态学和分子生物学鉴定,通过菌落及菌体形态特征、生理生化特性、16S r DNA序列测定结果,将该菌株鉴定为蜡样芽胞杆菌(Bacillus cereus)。Screening of strain resources for the production of microbial agents. In this study the insect anatomy tech-nology was used to draw out enteric duct in East Asia migratory locust （Locusta migratoria manilensis） , and isolatedthe bacterial strain with spreading dilution method, and using sodium carboxymethyl cellulose （CMC-Na） modifiedmedium to screen initially the cellulase producing strains. The results showed that a total of 12 intestinal strains wereseparated from the East Asia migratory locust that produce cellulase, all of them were bacteria, among them strainK005 with fairly high cellulase activity were studied on its morphology and molecular biology aspects of morphologicalfor its identification. The results through colony morphologic features, cell morphologic features, physiological and bi-ochemical specificities and 16S rDNA sequence analysis, the strain was identified as Bacillus cereus.

关 键 词：东亚飞蝗 肠道 纤维素酶 蜡样芽胞杆菌 

分 类 号：Q939.96[生物学—微生物学]