采用唾液淀粉酶活性指标对脾虚模型大鼠唾液采集方法的评价研究  

Evaluation of Saliva Collection Method in Rat Model of Spleen-deficiency by Using Salivary Alpha-amylase Activity Index

作　　者：林静[1,2] 卢群[1] 杨泽民[1] 

机构地区：[1]广东药科大学基础学院,广州510006 [2]嘉应学院医学院,广东梅州514031

出　　处：《中国中医基础医学杂志》2016年第7期909-911,924,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81102703)-基于AMY1基因拷贝数变异;N-糖基化和β-肾上腺素能受体介导的脾虚证唾液淀粉酶活性改变机制的研究;广东省科技计划项目(2013A032500005)-脾-物质代谢关联"新假说的miRNA物质基础及"从脾论治"代谢性疾病的分子机制研究";广东药学院科技处-附属第一医院联合自然科学培育基金项目(GYFYLH201303;GYFLH201313)

摘　　要：目的：评价脾虚模型大鼠唾液采集方法的可行性。方法：采集利血平所致脾虚组大鼠和正常组大鼠各24例酸刺激前后的唾液,其中刺激后的唾液以0.4 mol/l 0.50 cm×0.50 cm柠檬酸滤纸,每隔2.5 min刺激大鼠舌尖30 s,获得刺激后0~5 min、6~10 min和11~15 min的唾液。Bernfeld法测定唾液淀粉酶（s AA）活性,计算s AA活性比值。结果：脾虚组衰竭明显,体质量比正常组显著降低;正常组与分组前所有大鼠刺激前和刺激后s AA活性及其活性比值比较差异无统计学意义;脾虚组酸刺激前的s AA活性与正常组比较差异无统计学意义,酸刺激后3个时间段的s AA活性和活性比值均比正常组显著降低。结论：建立唾液采集方法经s AA活性分析,表现出与人较为一致的趋势,可以用于脾虚大鼠s AA相关研究。Objective To evaluate the feasibility of saliva collection method in rats with spleen-deficiency. Method Salivary samples from spleen-deficiency rats（ n = 24） induced by reserpine and normal rats（ n = 24） were collected before and after acid stimulation. Stimulated saliva of 0-5 min,6-10 min and 11-15 min were collected by putting 0. 4 mol / l 0. 50× 0. 50 cm of citric acid filter paper at the tongue tip 30 s every 2. 5 min. Salivary alpha-amylase（ s AA） activity was determined by the Bernfeld method,and s AA activity ratio was calculated. Result： Spleen-deficiency rats collapsed severely and their weight showed significantly lower than the normal rats. The s AA activity and s AA activity ratios had no significant difference between normal rats and all rats before be grouped. There was no significant difference in unstimulated s AA activity between spleen-deficiency and normal rats,while spleen-deficiency rats had lower s AA activity and s AA activity ratio in stimulated saliva of three time periods than normal rats. Conclusion Saliva collection method established by the present study,by which s AA activity and s AA activity ratio in rat showed similar trend with that of human,could be used in the research of spleen-deficiency rats related with s AA.

关 键 词：唾液淀粉酶 酸刺激 脾虚大鼠 唾液采集 

分 类 号：R285.5[医药卫生—中药学]