HPLC法测定地肤子中芦丁和槲皮素的含量  

Simultaneous determination of rutin and quercetin in kochiae fructus by HPLC

作　　者：肖作奇 文晓柯 欧阳波[1] 黄懿[1] 田霞[1] 潘涛[1] 

机构地区：[1]湖南省妇幼保健院药学部,湖南长沙410008

出　　处：《中国医药导报》2016年第21期146-148,152,共4页China Medical Herald

基　　金：湖南省中医药管理局科研项目(201671)

摘　　要：目的建立测定地肤子药材中芦丁和槲皮素含量的方法。方法采用高效液相色谱法结合二极管阵列检测器，选用AgilentTC—C18色谱柱，流动相为乙腈-0．2％磷酸（梯度洗脱），检测地肤子中芦丁和槲皮素的含量。结果芦丁和槲皮素的线性范围分别为0．0017-0．0532mg／mL和0．0011-0．0340mg／mL，平均加样回收率分别为98．5％、97．88％（RSD=1．57％、1．87％）。芦丁和槲皮素的平均含量分别为0．0592、0．0215mg／g。结论该方法简单易行，稳定性和重复性好，可用于地肤子中芦丁和槲皮素的含量测定。Objective To establish a method for simultaneous determination of rutin and quercetin in kochiae fructus. Methods High performance liquid chromatography was performed on the column of Agilent TC-C18 column with diode array detector. The mobile phase was acetonitrile-0.2% phosphoric acid solution （gradient elution）. The compositions of quantitative analysis were rutin and quercetin. Results The linear range of rutin and quercetin was 0.0017-0.0532 mg/mL and 0.0011-0.0340 mg/mL respectively. The average recoveries were 98.5% and 97.88% with RSD of 1.57% and 1.87% respectively. The average content of rutin and quercetin was 0.0592 mg/g and 0.0215 mg/g respectively. Con- clusion This method is suitable for the content determination of turin and quercetin in kochiae fructus with well stability and reproducibility.

关 键 词：地肤子 高效液相色谱 含量测定 芦丁 槲皮素 

分 类 号：R914.1[医药卫生—药物化学]