外源MeJA,SA及2种内生菌处理对白及幼苗生理及总酚含量影响  

Effects of exogenous Me JA,SA and two kinds of endophytic fungi on physiology and total phenols content of seedlings of Bletilla striata

作　　者：杨嘉伟[1] 王康才[1] 梁君怡 王娟[1] 夏天爽[1] 梁永富[1] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095

出　　处：《中国中药杂志》2016年第15期2794-2801,共8页China Journal of Chinese Materia Medica

摘　　要：通过施加外源Me JA,SA及2种内生菌处理,研究白及组培苗生理及总酚含量的影响。采用组培的方法将种子培养为无菌苗,再对其施加不同处理,观察及测定生理和总酚含量的变化。结果发现:SA各浓度处理下幼苗生长情况均较差,40μmol·L^(-1)Me JA,50 m L·L^(-1)Hypocrea koningii及10 m L·L^(-1)Trichoderma koningiopsis处理下幼苗生长状况较好;各浓度SA处理下,SOD,POD及CAT活性均较高,Me JA处理下SOD,POD在较高浓度时活性较高,CAT活性则在80μmol·L^(-1)时较高,H.koningii处理下SOD,POD活性均随处理浓度的升高而升高,CAT活性则在1 m L·L^(-1)时较高,T.koningiopsis处理下SOD,POD,CAT活性均随浓度升高先升高后降低,均在10 m L·L^(-1)时较高。4种处理下MDA、可溶性蛋白及脯氨酸含量均有不同程度的升高;60μmol·L^(-1)Me JA处理下幼苗多糖含量较高;40μmol·L^(-1)Me JA,60μmol·L^(-1)SA,1 m L·L^(-1)H.koningii及10 m L·L^(-1),T.koningiopsis处理下总酚含量较高。说明添加一定浓度的外源Me JA,SA及内生菌处理均能在一定程度上提高白及幼苗的抗逆性、提高总酚含量,一定浓度下Me JA及2种内生菌处理还具有促进幼苗生长的作用。Tissue culture seedlings of Bletilla striata were treated with MeJA, SA and two kinds of endophytic fungi in order to study the effects of those treatments on the physiology and total phenols content. The method of tissue culture was used to culture seeds into seedlings, and then different treatments were applied on them to observe and measure the changes of physiology and total phenols content. We find that the growth of seedlings treated with SA was poor, which treated with 40 μmol·L^-1 MeJA, 50 mL ·L^-1 Hypocrea koningii and 10 mL ·L^-1 Trichoderma koningiopsis showed better. The activity of SOD, POD and CAT was at a high level under SA treatment of each concentration. The activity of SOD and POD increased as the rise of MeJA concentration, while CAT was highest at 80 μmol·L^-1. The activity of SOD and POD increased with the increasing of the concentration of H. koningii treatment, while CAT reached the highest at 1 mL ·L^-1. The activity of SOD, POD and CAT increased first and then declined with the concentration of T. koningiopsis increasing, and the highest activity was at 10 mL ·L^-1. The contents of MDA, soluble protein and proline were increased more or less under the four treatments. The content of polysaccharide was at a high level under 60 μmol·L^-1 of MeJA. The total phenols content was at a high level under 40 μmol·L^-1 of MeJA, 60 μmol·L^-1 of SA, 1 mL ·L^-1 of H. koningii and 10 mL·L^-1 of T. koningiopsis. The results indicated that the addition of exogenous MeJA, SA and endophytic fungi under certain concentrations could improve the resistance of B. striata and increase the content of total phenols at some degree and the treatment of MeJA, 11. koningii and T. koningiopsis could promote the growth of seedlings under certain concentrations.

关 键 词：白及 Me JA SA HYPOCREA koningii Trichoderma koningiopsis 总酚 

分 类 号：S567.239[农业科学—中草药栽培]