基质金属蛋白酶基因启动区3个单核苷酸多态性与河南汉族群体慢性心力衰竭遗传易感性关联研究  

Correlation of matrix metalloproteinases promoter region three single nucleotide polymorphisms with genetic susceptibility of chronic heart failure in Henan Han population

作　　者：马圆真 刘亚静[1] 徐光华[1] 张倩[1] 王宇[1] 郭利伟[2] 黄艳梅[2] 

机构地区：[1]新乡医学院,河南新乡453003 [2]新乡医学院法医学系法医物证学教研室,河南新乡453003

出　　处：《新乡医学院学报》2016年第7期568-571,共4页Journal of Xinxiang Medical University

基　　金：河南省医学科技攻关计划项目(编号:201403131);河南省教育科学"十二五"规划立项重点课题(编号:2013-JKGHB-0035);新乡医学院大学生科研创新计划课题(编号:2014-21)

摘　　要：目的探讨基质金属蛋白酶(MMPs)基因启动区rs1799750、rs3025058及rs227610单核苷酸多态性(SNP)与河南汉族群体慢性心力衰竭(CHF)发病风险的相关性。方法采用限制性扩增片段长度多态性(PCRRFLP)方法检测124例CHF患者(CHF组)和144名健康者(对照组)的3个SNP,运用SHEsis软件分析数据。结果CHF组rs1799750的1G等位基因频率较对照组显著降低(P<0.01),1G1G基因型频率也较对照组显著降低(P<0.01)。CHF组1G6A单体型的频率显著低于对照组(P<0.01)。结论河南汉族群体中rs1799750位点1G等位基因和单体型1G6A可能会降低个体CHF的患病风险。Objective To investigate the relationship between single nucleotide polymorphism（ SNP） of rs1799750,rs3025058 and rs227610 at the promoter region of matrix metalloproteinases（ MMPs） genes and onset risk of chronic heart failure（ CHF） in Henan Han population. Methods Three SNP sites of 124 CHF patients（ CHF group） and 144 normal people（ control group） were detected by polymerase chain reaction restriction fragment length polymorphism（ PCR-RFLP）. And the data was analyzed by SHEsis soft. Results The frequency of 1G allele and 1G1 G genotype of rs1799750 of patients in the CHF group was significantly lower than that in the control group（ P 0. 01）. The frequency of 1G6 A haplotype of patients in CHF group was significantly lower than that in the control group（ P 0. 01）. Conclusion 1G allele of rs1799750 and haplotype 1G6 A may decrease the onset risk of CHF.

关 键 词：基质金属蛋白酶 慢性心力衰竭 单核苷酸多态性 限制性扩增片段长度多态性 

分 类 号：R541.6[医药卫生—心血管疾病]