长链非编码RNACCAT2在结肠癌组织的表达及其意义  

The expression of long noncoding RNA CCAT2 in colon cancer and its clinical significance

作　　者：卢震海[1] 林继宗[2] 彭健宏[1] 张荣欣[1] 区庆坚[1] 

机构地区：[1]中山大学肿瘤防治中心华南肿瘤学国家重点实验室肿瘤医学协同创新中心,广州510060 [2]中山大学附属第三医院肝胆外科,广州510630

出　　处：《中华实验外科杂志》2016年第8期2041-2043,共3页Chinese Journal of Experimental Surgery

基　　金：广东省科技计划项目（20128031800072）

摘　　要：目的检测长链非编码RNACCAT2（1ncRNACCAT：）基因在结肠癌中的表达并探讨其表达差异与临床资料及预后的关系。方法采用实时荧光定量聚合酶链反应（FQ．PCR）的方法检测结肠癌及其癌旁正常组织中lncRNACCAT2的表达量，取中位数分为高表达组与低表达组，分析其与临床资料及病例特征的关系；应用Kaplan-Meier法分析lncRNACCAT2的表达差异与临床预后的相关性。FQ—PCR法检测结肠癌细胞中lncRNACCAT：的表达，细胞计数试剂盒（CCK-8）法检测干扰lncRNACCAT：表达后结肠癌细胞增殖的变化。结果lncRNACCAT2在结肠癌中的相对表达水平为10．438±2．415，高于癌旁肠正常组织的相对表达水平（P〈0．01）；FQ-PCR高表达与肿瘤的大小、分化、血管浸润及肿瘤分期密切相关（P〈0．05）；lncRNACCAT：高表达组的中位生存时间为26个月，低表达组的中位生存时间为39个月，差异有统计学意义（P〈0．05）。lncRNACCAT2在48、72h的吸光度（A）值为0．16±0．02、0．28±0．01，均低于对照组的0．35±0．03、0．41±0．02，差异有统计学意义（P〈0．05）。结论lncRNACCAT：过表达参与了结肠癌的发生和发展。Objective To detect the expression of long chain non encoding CCAT2 RNA （lncRNA） gene in colon carcinoma and to investigate the relationship between the expression and clinical data and prognosis. Methods Real -time fluorescent quantitative polymerase chain reaction （FQ -PCR） method was used to detect the expression of long noncoding RNA CCAT2 in colon cancer and its adjacent normal tissues. The high expression group and low expression group were set up. The relationship between the expression of Kaplan - Meier and clinical data was analyzed. The correlation between the expression of long noncoding CCAT2 RNA and clinical prognosis was analyzed. FQ - PCR method was used to detect the expression of long noneoding RNA CCAT2 in colon cancer cell line, and the proliferation of colon cancer cells transfected with si - lncRNA CCAT2 was measured by cell counting kit - 8 （ CCK - 8） assay. Results FQ -PCR showed the higher expression of lncRNA CCAT2 in colon cancer （10. 438 ± 2. 415 ） than that in adjacent normal tissues （P 〈0. 01 ）. The high expression of FQ -PCR was closely related to tumor size, differentiation, vascular invasion and tumor stage （ P 〈 0. 05 ）. Median survival time in the lncRNA CCAT2 high expression group was significantly shorter than that in the low expression group （26 months vs. 39 months, P 〈 0. 05 ）. LncRNA CCAT2 was highly expressed in colon cancer cell line, and the down - regula- tion of it could inhibit the cell proliferation （48, 72 h： 0. 16 ±0.02, 0.28±0.01 vs. 0.35±0.03, 0.41±0. 02）. Conclusion LncRNA CCAT2 over expression is involved in the occurrence and develop- ment of colon cancer.

关 键 词：长链非编码RNA CCAT2 RNA 结肠癌 预后 

分 类 号：R735.35[医药卫生—肿瘤]