胸腺素β4在人脑胶质瘤组织中表达及意义的研究  

Expression and clinical significance of Thymosin β4 in human cerebral glioma

作　　者：解辉[1] 陆威成[2] 丁晓慧[1] 杨智航[3] 周慧[1] 丛敬[1] 王忠华[1] 杨志强[4] 

机构地区：[1]沈阳医学院组织胚胎学教研室,沈阳110034 [2]中国医科大学附属第一医院神经外科,沈阳110001 [3]沈阳医学院生理学教研室,沈阳110034 [4]沈阳医学院,沈阳110034

出　　处：《解剖科学进展》2016年第4期369-371,共3页Progress of Anatomical Sciences

基　　金：辽宁省科技厅博士启动基金(20131114);辽宁省教育厅科学技术研究一般项目(L2015535);沈阳医学院科技基金项目(20133053);沈阳医学院大学生科研立项(20141035)

摘　　要：目的探讨人脑胶质瘤组织中胸腺素β4基因和蛋白的表达及其临床意义。方法收集40例人脑胶质瘤患者手术肿瘤组织切除标本及2例癫痫病人手术后切除脑组织对照标本,采用逆转录聚合酶链反应法和免疫组织法检测所有标本中胸腺素β4基因和蛋白的表达。结果人脑胶质瘤组织中胸腺素β4m RNA和蛋白在各个级别胶质瘤组织中均有表达,而在正常脑组织表达缺失,不同病理级别胶质瘤之间胸腺素β4的m RNA和蛋白表达水平随着胶质瘤病理级别的增加而增加,表达水平均为I级<II级<III级<IV级。结论胸腺素β4基因和蛋白在人脑胶质瘤组织过表达,可能与其发生发展相关,可作为早期诊断的标志物。Objective To study the expression and clinical significance of thymosin β4 gene and protein in human cerebral glioma. Methods Fourty cases of human cerebral glioma specimens were collected, two normal brain tissues from internal decompression of patients with epilepsy were used as control. The expressions of thymosin β4 mRNA and protein were determined by RT-PCR and immunohistochemistry respectively. Results Thymosin β4 mRNA and protein were found to be expressed in all grades cerebral glioma, but not in normal brain tissue of patients with epilepsy. The expression levels of thymosin β4 mRNA and protein were increased as the pathological graxtes of gliomos increased, as followed： grade l〈gradeII〈grade III〈grade IV. Conclusion The overexpression of thymosin β4 mRNA and protein in human cerebral glioma indicates that Thymosin β4might be related to the occurrence and development of glioma and a marker for early diagnosis of glioma.

关 键 词：胸腺素Β4 人脑胶质瘤 病理级别 

分 类 号：R739.41[医药卫生—肿瘤]