二氯乙酸盐DCA对神经胶质瘤细胞U87影响的体外研究  

The effect of dichloroacetate on glioma U87 cells in vitro

作　　者：姚冰[1] 陈一[1] 史记[1] 张烨[1] 孙佩欣[1] 隋锐[1] 朴浩哲[1] 

机构地区：[1]中国医科大学肿瘤医院,辽宁省肿瘤医院神经外科,辽宁沈阳110042

出　　处：《解剖科学进展》2016年第4期396-399,共4页Progress of Anatomical Sciences

基　　金：辽宁省博士启动基金计划(20131059)

摘　　要：目的研究二氯乙酸盐(dichloroacetate,DCA)对神经胶质瘤细胞U87的生物学行为的影响及可能机制,探讨DCA与脑恶性肿瘤治疗之间的关系。方法神经胶质瘤细胞U87在DCA的干预下,通过MTT实验检测细胞生长能力,平板克隆形成实验检测细胞增殖能力,Western blotting检测caspase-3、bcl-2、bax、AC-tubulin的表达变化,荧光共聚焦实验进行AC-tubulin检测分析。结果 DCA对于U87细胞生长具有明显的抑制作用,抑制胶质瘤细胞克隆能力,进而抑制细胞增殖,促进U87细胞的凋亡,并且使AC-tubulin的表达明显增高,DCA使U87细胞阻滞在G0/G1期。结论 DCA抑制并杀伤胶质瘤细胞,在胶质瘤的未来治疗过程中,很可能成为治疗一类新药物。Objective To study the effect of dichloroacetate（DCA） on biological behivior and possible mechanism of glioma U87 cells in vitro. Methods Glioma U87 cells growth ability was measured by MTF, cell proliferation ability was measured by colony formation assay, Western blotting assay was used to detect the expressions of Caspase-3, Bcl-2, Bax and AC-tubulin, and the location of AC-tubulin under fluorescence confocal microscopy. Results DCA could inhibit U87 cells growth obviously, and promote U87 cells apoptosis by inhibiting glioma cell clone ability, upregulate the expressive level of AC-tubulin, enabling U87 cells stoping in the G0/G1 phase. Conclusion DCA has a significant inhibitory effect on the glioma cells, likely to be a new drug for the treatment of glioma.

关 键 词：DCA 神经胶质瘤 凋亡 

分 类 号：R739.41[医药卫生—肿瘤]