Six1和Ezrin在肾细胞癌组织中的表达及与疾病转归相关性研究  

Expression of Six1 and Ezrin genes in renal cell carcinoma and the correlation to disease prognosis

作　　者：曹学全[1] 杨朝晖[1] 顾华敏[1] 章辉[1] 卢洪胜[1] 范广民[1] 甘梅富 

机构地区：[1]台州恩泽医疗中心(集团)台州市中心医院,浙江台州318000 [2]台州恩泽医疗中心(集团)台州医院

出　　处：《中国预防医学杂志》2016年第7期527-531,共5页Chinese Preventive Medicine

基　　金：台州恩泽医疗中心(集团)科研基金项目(15EZD17)

摘　　要：目的研究Six1和Ezrin在肾细胞癌(renal cell carcinoma,RCC)组织中的表达及其临床意义。方法应用组织芯片技术、免疫组化En Vision法检测98例RCC和28例正常肾对照组织中Six1和Ezrin的表达情况,分析其与临床病理参数及预后的关系。结果 RCC组织中Six1和Ezrin的阳性表达率分别为71.43%(70/98)和76.53%(75/98),明显高于正常肾对照组织的14.29%(4/28)和17.86%(5/28),差异有统计学意义(P<0.05)。Six1、Ezrin表达率与RCC的病理分级及临床分期有关(P<0.05)。RCC组织中Six1和Ezrin蛋白表达呈正相关(P<0.05)。Log-rank分析结果显示,Six1和Ezrin表达与RCC患者的总生存率有关(P<0.05)。结论 RCC组织中Six1、Ezrin蛋白表达均升高,其表达与肾细胞癌的病理分级和临床分期密切相关,Six1和Ezrin表达与RCC患者预后有关。Objective To observe the expression and clinical significance of Six1 and Ezrin genes in renal cell carcinoma（RCC）. Methods The expression of Six1 and Ezrin in 98 renal cell carcinoma and 28 normal renal tissues was detected by tissue microarray technology and immunohistochemical method（En Vision）and was compared,the correlation to clinic-pahological factors and disease prognosis were analyzed as well. ResultsThe positive expressions of Six1 and Ezrin in RCC were 71.43% and 76.53%,respectively,which were significantly higher than those in normal renal tissue（P〈0.05）.The expression rates of Six1 and Ezrin were correlated to pathological grade and clinical stage（P〈0.05）.There was positive correlation between Six1 and Ezrin expression in renal cell carcinoma（P〈0.05）.Log-rank analysis revealed that the expressions of Six1 and Ezrin were closely related to the overall survival rate of cases with renal cell carcinoma（P〈0.05）. Conclusions Six1 and Ezrin are highly expressed in renal cell carcinoma,and the expression is closely correlated to pathological grade and clinical stage of renal cell carcinoma,as well as to disease prognosis.

关 键 词：肾细胞癌 Six1 EZRIN 预后 组织芯片 

分 类 号：R737.11[医药卫生—肿瘤]