组蛋白精氨酸甲基转移酶5在胃癌组织中的表达及其意义  

Expression of PRMT5 in human gastric cancer tissue and its significance

作　　者：孔桂美[1] 王继军[1] 陶文华[1] 卜平[1] 钱锋[1] 

机构地区：[1]扬州大学医学院微生物与免疫学教研室,江苏扬州225001

出　　处：《吉林大学学报（医学版）》2016年第4期753-756,I0004,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81272537;30801497);江苏省教育厅高校自然科学基金资助课题(11KJB360010);扬州大学"新世纪人才工程"资助课题(2012)

摘　　要：目的:探讨组蛋白精氨酸甲基转移酶5(PRMT5)在胃癌组织中的表达,阐明PRMT5与胃癌发生及临床病理学特征之间的关系,为胃癌的防治提供新的靶点。方法:43例人胃癌组织及15例癌旁正常胃组织样本分别作为胃癌组和对照组,采用免疫组织化学方法检测2组样本中PRMT5的高表达率。结果:与对照组(13.3%)比较,胃癌组胃癌组织中PRMT5高表达率(72.1%)明显升高(P<0.05)。对照组弱阳性表达标本中,棕黄色颗粒主要位于细胞浆中,表现为弱到中等强度表达,细胞核染色较少;高分化胃癌标本中,棕黄色颗粒主要位于细胞浆中,表现为中等和强表达,细胞核染色较多;中、低分化和未分化胃癌标本中棕黄色颗粒主要位于细胞浆和细胞核中,细胞核表现为强表达。PRMT5高表达率与肿瘤大小、分化程度、浸润深度、淋巴结转移情况及TNM分期有关联(P<0.05或P<0.01),与患者的性别和年龄无关联(P<0.05)。结论:PRMT5高表达可能对胃癌的发生发展起促进作用。Objective：To investigate the expression of protein arginine methyltransferase 5（PRMT5）in the gastric cancer tissue,and to explore the relationship between the PRMT5 expression and the occurrence and the clinicopathologic characteristics of gastric cancer,and to find a new treatment target for gastric cancer.Methods：Forty-three cases of gastric cancer tissue and 15 cases of adjacent normal gastric tissue were used as gastric cancer group and control group,respectively.The immunohistochemical staining was used to detect the expression of PRMT5 in the specimens.Results：Compared with control group（13.3%）,the expression rate of PRMT5 in gastric cancer group（72.1%）was significantly increased（P〈0.05）.In control group,there was some weak positive expression of PRMT5,the expressions were weak or moderate,the tan particles mainly located in the cytoplasm,less in the nuclei.In high differentiation gastric cancer specimens,the tan particles mainly located in the cytoplasm and more in nuclei,characterized by medium or strong expressions.In poorly differentiated and undifferentiated gastric cancer specimens,the tan particles mainly located in the cytoplasm and nucleus,and strongly expressed in nucleus.The higher expression of PRMT5 was associated with the size of tumor diameter,degree of differentition,depth of invasion,degree of lymph node metastasis,and TNM stage（P〈0.05 or P〈0.01）,but had no correlation with the gender and age of patients（P〈0.05）.Conclusion：High expression of PRMT5 may play an important role in the occurrence and development of gastric cancer.

关 键 词：胃肿瘤 组蛋白精氨酸甲基转移酶5 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]