诱导性多能干细胞治疗小鼠肝损伤的初步研究  

Primary study of induced pluripotent stem cells in treatment of liver injury in mice

作　　者：王建军[1] 赵平[1] 靳雪源[1] 程勇前[1] 刘鸿凌[2] 闫涛[1] 卿松[1] 丁宁[1] 辛绍杰[2] 

机构地区：[1]解放军第302医院国际肝病诊疗中心,北京100039 [2]解放军302医院肝衰竭诊疗与研究中心,北京100039

出　　处：《中国肝脏病杂志（电子版）》2016年第2期39-43,共5页Chinese Journal of Liver Diseases：Electronic Version

基　　金：军队十二五重大课题(BWS11J075)

摘　　要：目的了解诱导性多能干细胞(induced pluripotent stem cells,IPS细胞)对肝细胞损伤的治疗作用。方法取健康雄性C57小鼠28只,分为健康对照组、实验组和实验对照组。实验组小鼠腹腔内注射2500 mg/kg氨基半乳糖造小鼠肝功能损伤模型,造模2天后在实验组小鼠尾静脉注射CFSE荧光标记后的IPS细胞。在注射IPS细胞后5天、10天、15天和20天时,健康对照组、实验组、实验对照组各取两只小鼠处死,取肝脏标本,分别行冰冻切片显微镜观察荧光,石蜡切片行HE染色,取血行生物化学检查。结果IPS细胞注射入小鼠体内后,可在小鼠肝脏定植,注射IPS细胞的小鼠血中ALT、AST下降速度较未注射IPS细胞的小鼠明显加快。肝脏病理损伤恢复情况较未注射IPS细胞的小鼠明显改善。结论 IPS细胞注射入肝功能损伤C57小鼠体内,可对小鼠的肝功能损伤有一定的修复作用。Objective To investigate the therapeutic effect of induced pluripotent stem cells（IPS cells） on liver injury. Methods Total of 28 healthy male C57 mice were divided into healthy control group, experimental group and experimental control group. In the experimental group, a mouse liver injury model was induced by intraperitoneal injection of amino galactose（2500 mg/kg）. After two days, the IPS cells were injected into the mice in the experimental group with fluorescence labeled CFSE. After 5 days, 10 days, 15 days and 20 days, the mice in healthy control group, experimental group and experimental control group were sacrificed and the liver samples were taken for frozen section and paraffin sections were stained with HE staining. The liver function were detected. Results The IPS cells can be settled in the liver of the mice after injection. ALT and AST in the blood of mice after injecting with IPS cells decreased significantly faster than those in the non IPS cells and the recovery of liver pathological injury improved significantly. Conclusion IPS cells can repair the damage of liver after injected into the C57 mice with liver injury.

关 键 词：诱导性多能干细胞 肝功能损伤 

分 类 号：R575[医药卫生—消化系统]