可溶性鸟苷酸环化酶抑制剂对体外培养人胚胎眼巩膜成纤维细胞胶原合成的影响  

The regulation of human fetal scleral fibroblasts using soluble guanylyi cyclase antagonist in vitro

作　　者：李创[1] 吕勇[2] 赵宏[1] 

机构地区：[1]河南省郑州市第二人民医院眼科,450006 [2]郑州大学第一附属医院眼科,450003

出　　处：《中华眼视光学与视觉科学杂志》2016年第7期415-419,共5页Chinese Journal Of Optometry Ophthalmology And Visual Science

摘　　要：目的研究可溶性鸟苷酸环化酶（sGC）抑制剂（NS．2028）对人胚胎眼巩膜成纤维细胞（HFSFs）的增殖、I型胶原蛋白（COL1A1）合成的影响。方法实验研究。体外培养的HFSFs分为2组，实验组加入NS．2028，分为0．1、1．0、10．0、100．0μmol／L4个浓度梯度；对照组加入与实验组对应的等体积细胞培养液。分别采用CCK8法、荧光定量PCR法、免疫荧光法检测对照组和实验组HFSFs吸光度（OD）值、COL1A1mRNA相对表达量及COL1A1的表达。数据采用单因素方差分析处理。结果CCK8法检测对照组HFSFs的OD值为0．82±0．04，实验组在4个浓度梯度NS．2028刺激下，OD值分别为0．81±0．05、0．83±0．05、0．85±0．05、0．81±0．05，与对照组相比差异无统计学意义（F=0．620，P〉0．05）。荧光定量PCR法检测实验组在4个浓度梯度NS．2028刺激下，COL1A1 mRNA相对表达量分别为：1．06±0．75、1．19±0．68、1．40±0．18、1．88±0．24，与对照组相比相对表达量增高，差异有统计学意义（F=32．138，P〈O．05）；免疫荧光法检测实验组在4个浓度梯度NS．2028刺激下，与对照组相比其COL1Al的分泌量显著增高。结论NS．2028在促进HFSFsCOL1A1合成方面发挥显著作用．对HFSFs的增殖可能没有影响。NS-2028可能在一定程度上能减缓近视的发展。Objective To study scleral fibroblast proliferation and type I collagen compound in human fetal scleral fibroblasts （HFSFs） by applying the soluble guanylyl cyclase （sGC） antagonist （NS-2028）. Methods Experimental study. HFSFs were divided into two groups. The experimental group was treated with NS-2028; the control group was not treated. The experimental concentrations were 0.1, 1.0, 10.0, 100.0 μmol/L. After NS-2028 stimulation, the changes in the HFSFs of the control group and experimental groups were analyzed with CCK8, realtime fluorescence quantitative PCR （RTFQ PCR） and immunofluorescence （IF）. Results （1）The OD value of HFSFs in the control group was 0.82±0.04. The OD values of the four NS-2028 treated groups were 0.81±0.05, 0.83±0.05, 0.85± 0.05 and 0.81±0.05, there were no significant differences from the control group （F=0.620, P〉0.05）. （2）The relative transcript levels of COL1A1 mRNA in the the four NS-2028 treated groups were 1.06±0.75, 1.19±0.68, 1.40±0.18, 1.88±0.24, there were significant differences compared to the control group （F=32.138, P〈0.05）. （3）The relative expression level of COL1A1 in HFSFs increased in the four NS-2028 treated groups compared to the control group. Conclusion NS-2028 plays a significant role in promoting the composition of COL1A1. There may be no significant difference in the proliferation of HFSFs. However, COL1A1 may directly affect HFSFs, delaying the development of myopia.

关 键 词：成纤维细胞 胶原I型 细胞增殖 可溶性鸟苷酸环化酶抑制剂 

分 类 号：R778.11[医药卫生—眼科]