Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus  

作　　者：Cuiqing Zhong Meiling Zhang Qi Yin Han Zhao Yang Wang Sexin Huang Wenrong Tao Keliang Wu Zi-Jiang Chen Jinsong Li 

机构地区：[1]State Key Laboratory of Cell Biology, CAS Center for Excellence in Mo- lecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China [2]Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China [3]College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China [4]Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200127, China [5]Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China National Research Center for Assisted Reproductive Technology and Reproductive Genetics, China The Key laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, Shandong 250021, China

出　　处：《Cell Research》2016年第6期743-746,共4页细胞研究（英文版）

基　　金：Acknowledgments We thank W Bian, X Wang and F Zhang from Cell Biology Facility for support with cell sorting, Y Chen and X Ding from the Stem Cell Core Facility at SIBCB for support with cell cultures, H Li, M Li, H Deng, R Hou and H Xiao from Shanghai Biochip Company for sequencing data analysis, and Y Jin and Y Zeng from SIBS for providing antibodies. This study was supported by the Ministry of Science and Technology of China （2014CB964803, 2012CB944700, 2015AA020307 and 2013CB967103） and the National Natural Science Foundation of China （91319310, 31225017, 31530048, 81430029, 81490743, 31371453 and 31571548）.

摘　　要：Haploid embryonic stem cells （haESCs） have been recently generated from parthenogenetic （PG） or andro- genetic （AG） blastoeysts of different mammals, including mouse, rat and monkey, enabling genetic screening at both cellular and organism levels [1-6]. However, whether haESCs can be generated from human remains unknown. In this study, we establish protocols for the derivation of stable haESCs from human PG haploid blastocysts obtained by microsugical removal of male pronucleus from fertilized eggs. Human haESCs are pluripotent and sustain typical maternal imprinting status.

关 键 词：期刊 编辑工作 规范 写作方法 

分 类 号：G232[文化科学]