AP-2α对肺腺癌A549细胞MnSOD表达的作用研究  

Study on the role of AP-2α in the expression of Mn SOD in the lung cancer A549 cells

作　　者：杨欢[1] 刘志光[1] 周锐[2] 

机构地区：[1]湖南师范大学第一附属医院湖南省人民医院呼吸内科,长沙410005 [2]中南大学湘雅二医院呼吸内科,长沙410011

出　　处：《临床与病理杂志》2016年第7期919-923,共5页Journal of Clinical and Pathological Research

基　　金：国家临床重点专科建设项目~~

摘　　要：目的:探讨转录因子激活蛋白-2α(activator protein-2 alpha,AP-2α)对锰超氧化物歧化酶(manganese superoxide dismutase,Mn SOD)m RNA和蛋白表达的影响,探讨AP-2α下调Mn SOD表达的可能的分子机制。方法:转染Ad-AP-2α和AP-2αsi RNA进入A549细胞,上调和下调AP-2α后,采用实时定量RTPCR及Western blot检测A549细胞内Mn SOD的m RNA及蛋白质的表达水平。结果:1)Ad-AP-2α转染A549细胞后,Mn SOD m RNA和蛋白质表达均下降;2)AP-2αsi RNA转染A549细胞后,Mn SOD m RNA及蛋白质表达均增加。结论:AP-2α对肺腺癌A549细胞Mn SOD的m RNA和蛋白表达起下调作用。Objective： To investigate the effect of AP-2α on MnSOD gene/protein expression in lung cancer （A549） cells and its possible molecular mechanism of downregulation.Methods： A549 cells were transfected with adenovirus vector overexpressing AP-2α and AP-2α siRNA knockdowning AP-2α. The levels of AP-2α on MnSOD gene/protein expression were detected by RT-PCR and western blot.Results： 1） Atfer transfected with Ad-AP-2α in A549 cells, the levels of MnSOD mRNA and protein expression both decreased; 2） the expression of mRNA and protein of MnSOD increased atfer AP-2α siRNA transfected in A549 cells.Conclusion：hTe results revealed that AP-2α can effectively down-regulate MnSOD gene/protein expression.

关 键 词：激活蛋白-2 锰超氧化物歧化酶 肺癌 AP-2 

分 类 号：R734.2[医药卫生—肿瘤]