ISSR分子标记鉴定巴马香猪  

Species Authentication of Bama Miniature Pig through Inter-simple Sequence Repeat

作　　者：金玉兰[1] 李婉丽[2] 李松[2] 李玺洋[2] 杨阳[2] 陈秋虹[3] 孙群[2] 

机构地区：[1]四川大学轻纺与食品学院,成都610064 [2]四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610064 [3]广西壮族自治区分析测试研究中心,南宁530022

出　　处：《四川动物》2016年第4期569-573,共5页Sichuan Journal of Zoology

基　　金：广西科学研究与技术开发计划项目(12118011-2B)

摘　　要：结合线粒体基因和ISSR两种DNA分子标记技术对巴马香猪、环江香猪、藏猪、巴马本地土猪和成华猪进行种类鉴定。研究从55条ISSR引物中筛选出1条带型清晰、重复性好的引物53可以鉴别巴马香猪、环江香猪、藏猪、巴马本地土猪和成华猪。巴马香猪与藏猪线粒体基因分析结果表明,藏猪具有特异的短串联重复片段"TGTGTACGCA",可用于进一步鉴别巴马香猪与藏猪。ISSR分子标记与聚类分析结果显示,引物53可以鉴别5种猪并成功鉴定了一份未知种类的巴马产猪肉样品。结果表明,ISSR分子标记与线粒体基因联合分析可以快速准确鉴别巴马香猪、环江香猪、藏猪、巴马本地土猪和成华猪。In this study,species identification of Bama miniature pig was carried out by DNA fingerprinting of meats from Tibetan pig,Huanjiang miniature pig,Bama native pig,and Chenghua pig through inter-simple sequence repeat（ ISSR）and mitochondrial DNA（ mt DNA） analysis. One clear and repeatable primer pair named No. 53 was selected from 55 primers,which successfully separated Bama miniature pig from the other four pigs. To further differentiate Bama miniature pig and Tibetan pig,specific tandem repeat sequence of Tibetan pig‘TGTGTACGCA＇was found out in the mt DNA. According to the results of ISSR and UPGMA cluster analysis,primer No. 53 was able to attribute to the identification of the five different pigs and one unknown pig. Therefore,ISSR coupled with UPGMA and mt DNA analysis represented an effective tool in species identification.

关 键 词：巴马香猪 鉴定 线粒体基因 ISSR 

分 类 号：S828[农业科学—畜牧学]