颅脑创伤后期大鼠代谢型谷氨酸受体5表达变化及牛磺酸治疗作用  

The change of metabotropic glutamate receptor 5 expression level in rats with latestage traumatic brain injury and the therapeutic effect of taurine

作　　者：蔡英[1] 黄慧玲[1] 范维佳[1] 武俏丽[1] 

机构地区：[1]天津市环湖医院天津市神经外科研究所天津市脑血管与神经变性重点实验室,300350

出　　处：《中国现代神经疾病杂志》2016年第8期503-508,共6页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：国家自然科学基金资助项目(项目编号:30973089);国家自然科学基金资助项目(项目编号:81571216);天津市自然科学基金重点项目(项目编号:16JCZDJC35500);天津市应用基础与前沿技术研究计划(项目编号:13JCYBJC23200);天津市卫生局科技基金资助项目(项目编号:2012KZ034)~~

摘　　要：目的探讨颅脑创伤后期(第7天)大鼠脑组织代谢型谷氨酸受体5(m Glu R5)表达变化,以及牛磺酸治疗作用。方法液压脑损伤打击仪制备液压打击颅脑创伤大鼠模型,采用随机数字表法将30只无特定病原体级Sprague-Dawley大鼠随机分为对照组、颅脑创伤组和牛磺酸治疗组(各10只),干湿重法检测大鼠脑组织含水量,实时荧光定量聚合酶链反应和Western blotting法检测水通道蛋白4(AQP4)和m Glu R5 m RNA和蛋白表达变化。结果与对照组相比,颅脑创伤组大鼠脑组织含水量(t=4.893,P=0.002)、AQP4 m RNA(t=6.523,P=0.000)和蛋白(t=4.366,P=0.008)表达水平升高,m Glu R5m RNA(t=5.776,P=0.001)和蛋白(t=3.945,P=0.014)表达水平降低;经牛磺酸治疗后,大鼠脑组织含水量(t=2.151,P=0.140)、AQP4 m RNA(t=1.144,P=0.432)和蛋白(t=0.367,P=0.804)降至正常水平,m Glu R5 m RNA(t=1.824,P=0.216)和蛋白(t=1.185,P=0.414)升至正常水平。相关分析显示,脑组织含水量与m Glu R5 m RNA(r=-0.617,P=0.014)和蛋白(r=-0.665,P=0.007)呈负相关,与AQP4蛋白呈正相关(r=0.658,P=0.008)。结论牛磺酸可以升高颅脑创伤后期(第7天)大鼠脑组织m Glu R5表达水平,降低脑水肿和脑组织含水量,具有抑制性神经递质作用。Objective To investigate the change of metabotropic glutamate receptor 5 （mGluRS） expression level in rats with late-stage （the 7th day） traumatic brain injury （TBI） and the role of taurine. Methods The left cerebral TBI rat models were made by using lateral fluid percussion method. A total of 30 specific pathogen free （SPF） male Sprague-Dawley （SD） rats were randomly divided into 3 groups： sham operation group （control group）, TBI model group （TBI group） and taurine treatment group （taurine group）. Wet and dry weight method was used to measure the brain water content. Real- time fluorescent quantitative polymerase chain reaction （PCR） and Western blotting were used to detect the change of mRNA and protein expression of aquaporin 4 （AQP4） and mGluR5 in each group. Results Compared with control group, the brain water content （t = 4.893, P = 0.002）, AQP4 mRNA （t = 6.523, P = 0.000） and protein （t = 4.366, P = 0.008） expression were upregulated, while mGluR5 mRNA （t = 5.776, P = 0.001） and protein （t = 3.945, P = 0.014） expression were downregulated in TBI group. After taurine treatment, the brain water content （t = 2.151, P = 0.140）, AQP4 mRNA （t = 1.144, P = 0.432） and protein （t = 0.367, P = 0.804） decreased to normal, while mGluR5 mRNA （t = 1.824, P = 0.216） and protein （t = 1.185, P = 0.414） increased to normal Correlation analysis showed brain water content was negatively correlated with mGluR5 mRNA （r =- 0.617, P = 0.014） and mGluR5 protein （r =- 0.665, P = 0.007）, while it was positively correlated with AQP4 protein （r = 0.658, P = 0.008）. Conclusions Taurine can significantly increase the mGluR5 expression level of brain issue in the late-stage （the 7th day） of TBI and decline brain edema and brain water content. It may be a potential protective agent as an inhibitory neurotransmitter.

关 键 词：颅脑损伤 受体 谷氨酸 牛磺酸 疾病模型 动物 

分 类 号：R651.15[医药卫生—外科学]