Protective effects of ginsenoside Rg1 against hydrogen peroxide-induced injury in human neuroblastoma cells  

作　　者：Zhi-gao Sun Li-ping Chen Fa-wei Wang Cheng-yong Xu Miao Geng 

机构地区：[1]Department of Traditional Chinese Medicine,Hainan Branch Hospital of Chinese PLA General Hospital [2]Department of Traditional Chinese Medicine,Chinese PLA General Hospital [3]Institute of Gerontology,Chinese PLA General Hospital

出　　处：《Neural Regeneration Research》2016年第7期1159-1164,共6页中国神经再生研究（英文版）

基　　金：supported by the Research and Development Project Fund of Science and Technology Plan Program of Science and Technology Bureau of Beijing of China,No.Z111107067311022

摘　　要：The active ingredient of ginseng,ginsenosides Rg1,has been shown to scavenge free radicals and improve antioxidant capacity.This study hypothesized that ginsenosides Rg1 has a protective role in human neuroblastoma cells injured by H2O2.Ginsenosides Rg1 at different concentrations（50 and 100 μM） was used to treat H2O2（150 μM）-injured SH-SY5 Y cells.Results demonstrated that ginsenoside Rg1 elevated the survival rate of SH-SY5 Y cells injured by H2O2,diminished the amount of leaked lactate dehydrogenase,and increased superoxide dismutase activity.Ginsenoside Rg1 effectively suppressed caspase-3 immunoreactivity,and contributed to heat shock protein 70 gene expression,in a dose-dependent manner.These results indicate that ginsenoside Rg1 has protective effects on SH-SY5 Y cells injured by H2O2 and that its mechanism of action is associated with anti-oxidation and the inhibition of apoptosis.The active ingredient of ginseng,ginsenosides Rg1,has been shown to scavenge free radicals and improve antioxidant capacity.This study hypothesized that ginsenosides Rg1 has a protective role in human neuroblastoma cells injured by H2O2.Ginsenosides Rg1 at different concentrations（50 and 100 μM） was used to treat H2O2（150 μM）-injured SH-SY5 Y cells.Results demonstrated that ginsenoside Rg1 elevated the survival rate of SH-SY5 Y cells injured by H2O2,diminished the amount of leaked lactate dehydrogenase,and increased superoxide dismutase activity.Ginsenoside Rg1 effectively suppressed caspase-3 immunoreactivity,and contributed to heat shock protein 70 gene expression,in a dose-dependent manner.These results indicate that ginsenoside Rg1 has protective effects on SH-SY5 Y cells injured by H2O2 and that its mechanism of action is associated with anti-oxidation and the inhibition of apoptosis.

关 键 词：ginsenoside neuroblastoma peroxide injured protective caspase dehydrogenase suppressed manner antioxidant 

分 类 号：R739.4[医药卫生—肿瘤]