Differential temporal expression of matrix metalloproteinases following sciatic nerve crush  

作　　者：Jing Qin Guang-bin Zha Jun Yu Hong-hong Zhang Sheng Yi 

机构地区：[1]Jiangsu Key Laboratory of Neuroregeneration,Co-innovation Center of Neuroregeneration,Nantong University

出　　处：《Neural Regeneration Research》2016年第7期1165-1171,共7页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Foundation of Jiangsu Province of China,No.BK20150409;the Natural Science Foundation of Jiangsu Higher Education Institutions of China,No.15KJB180013;the Scientific Research Foundation of Nantong University in China,No.14R29;the Natural Science Foundation of Nantong City in China,No.MS12015043;the Priority Academic Program Development of Jiangsu Higher Education Institutions in China

摘　　要：We previously performed transcriptome sequencing and found that genes for matrix metalloproteinases（MMPs）,such as MMP7 and 12,seem to be highly upregulated following peripheral nerve injury,and may be involved in nerve repair.In the present study,we systematically determined the expression levels of MMPs and their regulators at 1,4,7 and 14 days after sciatic nerve crush injury.The number of differentially expressed genes was elevated at 4 and 7 days after injury,but decreased at 14 days after injury.Among the differentially expressed genes,those most up-regulated showed fold changes of more than 214,while those most down-regulated exhibited fold changes of more than 2-10.Gene sequencing showed that,at all time points after injury,a variety of MMP genes in the “Inhibition of MMPs” pathway were up-regulated,and their inhibitor genes were down-regulated.Expression of key up-and down-regulated genes was verified by quantitative real-time polymerase chain reaction analysis and found to be consistent with transcriptome sequencing.These results suggest that MMP-related genes are strongly involved in the process of peripheral nerve regeneration.We previously performed transcriptome sequencing and found that genes for matrix metalloproteinases（MMPs）,such as MMP7 and 12,seem to be highly upregulated following peripheral nerve injury,and may be involved in nerve repair.In the present study,we systematically determined the expression levels of MMPs and their regulators at 1,4,7 and 14 days after sciatic nerve crush injury.The number of differentially expressed genes was elevated at 4 and 7 days after injury,but decreased at 14 days after injury.Among the differentially expressed genes,those most up-regulated showed fold changes of more than 214,while those most down-regulated exhibited fold changes of more than 2-10.Gene sequencing showed that,at all time points after injury,a variety of MMP genes in the “Inhibition of MMPs” pathway were up-regulated,and their inhibitor genes were down-regulated.Expression of key up-and down-regulated genes was verified by quantitative real-time polymerase chain reaction analysis and found to be consistent with transcriptome sequencing.These results suggest that MMP-related genes are strongly involved in the process of peripheral nerve regeneration.

关 键 词：sequencing crush systematically regeneration verified repair TIMP differentially enriched degrade 

分 类 号：R745[医药卫生—神经病学与精神病学]