协同刺激因子B7-H3和B7-H4在不同临床分期胃癌组织中的表达  

Expression of costimulatory molecules B7-H4 and B7-H3 in different clinical stages of gastric carcinoma

作　　者：贾业贵[1] 许浪[1] 

机构地区：[1]湖北省江汉大学附属医院消化内科,湖北武汉430000

出　　处：《热带医学杂志》2016年第7期847-849,868,共4页Journal of Tropical Medicine

摘　　要：目的探讨协同刺激因子B7-H3、B7-H4在临床不同分期的胃癌组织中的表达及其临床意义。方法选取2013-2015年湖北省江汉大学附属医院157例胃癌组织,分别应用RT-PCR检测胃癌组织、胃癌细胞株BGC-823中B7-H4和B7-H3 mRNA的表达,Western Blot检测B7-H4和B7-H3蛋白的表达。胃癌患者不同临床特征与B7-H3、B7-H4表达水平的相关性采用χ-2检验,不同临床特征患者死亡率的比较采用Kaplan-Meier法及Log-rank检验。结果B7-H4、B7-H3在胃癌组织中的mRNA表达水平相对于癌旁组织呈现高表达。B7-H4在临床分期越高的组织中呈现高表达,而B7-H3在临床分期越高的组织中呈现低表达趋势。恶性程度越高的胃癌组织中,B7-H4的mRNA水平及蛋白水平越高,B7-H3的表达水平越低。高表达B7-H4且低表达B7-H3与胃癌患者预后显著相关（P〈0.05）。结论协同刺激分子B7-H4和B7-H3在胃癌中的表达水平与临床病理因素及预后密切相关,对该两分子的检测在胃癌诊断和预后判断中具有潜在的临床应用价值。Objective To investigate the expression levels and clinical significance of costimulatory molecules B7-H4 and B7-H3 in different clinical stages of gastric carcinoma. Methods 157 cases of gastric cancer tissue samples were collected from 2013 to 2015 in our hospital. The expression of B7-H4 mRNA and B7-H3 mRNA in gastric carcinoma tissues and BGC-823 cells were detected and quantified by RT-PCR. The expression of B7-H4 protein and B7-H3 protein were detected by Western Blot. The correlation between different expression levels of B7-H3 and B7-H4 and clinical characteristics were analyzed by chi-square test. Survival rates of different clinical and pathological characteristics were compared using Kaplan-Meier arid Log-rank test. Results B7-H4, B7-H3 mRNA expression levels in gastric cancer tissue present highly expressed relative to tissue adjacent to carcinoma. B7-H4 in clinical stage, the higher organizations present high expression, and the lower organizations, relative to the clinical stages B7-H3 in clinical stage, the higher the group showed a trend of lower expression.The m RNA and protein levels of B7-H4 were higher in the advance stages of gastric carcinoma, but the B7-H3 had lower expression levels. High expression of B7-H4 and low expression of B7-H3 were significantly related to the prognosis of gastric cancer（P〈0.05）. Conclusion Expression of costimulatory molecules B7-H4 and B7-H3 in gastric carcinoma is significantly correlated with the clinicopathological parameters and postoperative prognosis of patients. B7-H4 and B7-H3 may have a potential role in clinical diagnosis and prognostic evaluation of gastric carcinoma.

关 键 词：胃癌 B7-H4 B7-H3 协同刺激因子 

分 类 号：R735.2[医药卫生—肿瘤]