鼻咽癌颈淋巴结转移相关甲基化基因的筛选  

Screening for Cervical Lymph Node Metastasis-related Epigenetically Masked Genes in Nasopharyngeal Carcinoma

作　　者：邹桂年 邱前辉[2] 叶宇东 严雪原 

机构地区：[1]广东省龙川县人民医院耳鼻咽喉科,广东龙川517300 [2]广东省人民医院//广东省医学科学院耳鼻咽喉头颈外科,广东广州510080 [3]广东省东莞大朗医院耳鼻咽喉科,广东东莞523770

出　　处：《中山大学学报（医学科学版）》2016年第4期530-534,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：中国癌症基金会项目(S2013014);广东省医学科学技术研究基金项目(WSTJJ20131225440982197202101414)

摘　　要：【目的】分析鼻咽癌全基因组基因甲基化状态,筛选鼻咽癌颈淋巴结转移相关的甲基化基因。【方法】收集I期(T1N0M0)鼻咽癌石蜡标本及有颈部淋巴结转移的鼻咽癌组织标本(T1-4N1-3M0)各4例,分别提取DNA,富集两组样品基因组甲基化片段后采用甲基化芯片对两组标本进行高通量测序分析,对杂交信号进行扫描及初步数据处理,分析并筛选出两组标本的差异甲基化基因。【结果】Nimble Scan v2.5软件比较分析两组标本数据显示33个基因甲基化具有差异性,提示此33个基因可能为有颈部淋巴结转移鼻咽癌患者的甲基化基因谱。【结论】初步筛选鼻咽癌颈部淋巴结转移相关的33个甲基化基因,而DNA甲基化芯片是筛选异常甲基化基因的有效方法。[Objective] To investigate the status of DNA methylation in nasopharyngeal carcinoma （NPC） for screening out the cervical lymph node metastasis-related epigenetically masked genes. [ Methods ] Four pairs of specimens of stage I NPC and NPC with cervical lymph node metastasis were collected to extracted DNA ; The enriched genomic methylation fragments of two group specimens were used for high-throughput sequencing analysis by NimbleGen Human DNA Methylation 3 ~ 720 K CpG Island Plus RefSeq Promoter Array. We scanned the hybridization signal, processed preliminary data and identified the differentiated hypermethylated genes between the two groups NPC specimens. [ Results ] 33 methylated genes displayed significance of difference between the data of two groups by comparative analysise. It imply that they might be the lymph node metastasis-associated DNA methylation profiles in NPC patients. [Conclusion] 33 novel cervical lymph node metastasis-related epigenetically masked genes of NPC were preliminary verified and high density DNA methylation microarrays is indentified as an effective method for screening aberrantly methylated genes.

关 键 词：鼻咽癌 淋巴结转移 基因 甲基化 基因芯片 

分 类 号：R73[医药卫生—肿瘤]