炭样小单孢菌中抗生素生物合成相关蛋白的筛选  

Screening of the Protein Related to Antibiotics Biosynthesis in Micromonospora carbonacea JXNU-1

作　　者：陈娟[1] 杨慧林[1] 黄运红[1] 龙中儿[1] 

机构地区：[1]江西师范大学生命科学学院,南昌330022

出　　处：《中国生物工程杂志》2016年第7期55-63,共9页China Biotechnology

基　　金：国家自然科学基金(31160029;31360018);江西省自然科学基金(20132BAB204007;20161BAB204174)资助项目

摘　　要：目的:筛选一株具有广谱抗菌活性的炭样小单孢菌JXNU-1中核苷类抗生素生物合成相关蛋白。方法:通过i TRAQ定量蛋白质组学技术对JXNU-1菌体生长期(36h)和产物合成期(108h)的差异蛋白进行鉴定和功能分析。结果:基于i TRAQ定量蛋白质组学技术共鉴定出炭样小单孢菌总蛋白质2 390个,差异表达蛋白172个,在产物合成期(108h)表达上调76个、表达下调96个。通过蛋白GO和COG注释等功能分析,筛选出12个与抗生素合成密切相关蛋白和5个生物合成基因簇。结论:利用i TRAQ技术筛选出炭样小单孢菌JXNU-1的抗生素合成相关蛋白,为阐明该抗生素的生物合成机制奠定实验依据。Objective： To screen the protein related to antibiotics biosynthesis in Micromonospora carbonacea JXNU-1 with broad-spectrum antimicrobial activity. Methods： i TRAQ was used to analyze the differentially expressed proteins between the time of antibiotics secretion / non-secretion in M. carbonacea JXNU-1. Results： A total of 2 390 proteins were identified using i TRAQ,and 172 proteins were differentially expressed during the antibiotics secretion time in M. carbonacea JXNU-1,including 76 up-regulated and 96 down-regulated. Twelve proteins and five gene clusters closely related to biosynthesis of antibiotics were screened based on the functional analysis of the differentially expressed proteins with GO and COG,annotation. Conclusion： The proteins related to antibiotics biosynthesis in M. carbonacea JXNU-1 have been screened based on i TRAQ,it could provide the theoretical basis for revealing the mechanism of antibiotics biosynthesis in M. carbonacea JXNU-1.

关 键 词：炭样小单孢菌 蛋白质组学 抗生素 生物合成途径 基因簇 

分 类 号：Q591.2[生物学—生物化学]