中国炎症性肠病患者分离到黏附侵袭性大肠埃希菌  

Adherent-invasive Escherichia coli isolated from the patient with inflammatory bowel disease in China

作　　者：曹卉[1] 卢锐锋[1] 陈立平[1] 周瑞[1] 刘静[1] 赵秋[1] 

机构地区：[1]武汉大学中南医院消化科,430071

出　　处：《中华消化杂志》2016年第7期481-485,共5页Chinese Journal of Digestion

摘　　要：目的了解黏附侵袭性大肠埃希菌的黏附力、侵袭率，以及在巨噬细胞中存活和增殖能力。方法对 IBD 患者回肠、结肠、直肠部位的黏膜组织进行细菌培养、分离、鉴定，PCR 法鉴定毒力基因。细菌与细胞的共培养后观察菌株的黏附力和侵袭力，以及在巨噬细胞中的存活和增殖能力。结果从肠黏膜标本中分离出5株黏附侵袭性大肠埃希菌。5株细菌2A、2B、15A、15B、19B 对Caco-2细胞的黏附率依次为（1．4±1．3）％、（1．5±0．6）％、（0．6±0．1）％、（1．4±0．4）％和（1．1±0．8）％；对 Int-407细胞的黏附率分别为（1．0±0．8）％、（1．5±0．8）％、（1．0±0．8）％、（1．0±0．8）％和（0．3±0．0）％。对Hep-2细胞侵袭率依次为（10．1±7．0）％、（0．7±0．4）％、（0．4±0．3）％、（2．2±1．0）％和（2．1±1．8）％；对 Int-407细胞的侵袭率分别为（0．7±0．5）％、（0．5±0．3）％、（2．8±1．2）％、（0．7±0．5）％和（0．5± 0．4）％。5株黏附侵袭性大肠埃希菌都能在吞噬细胞内存活和增殖。结论首次在中国 IBD 患者中检出5株黏附侵袭性大肠埃希菌，且均能在吞噬细胞内存活和增殖。Objective To study adhesion and invasion of adherent-invasive Escherichia coli strains and its ability of survival and proliferation in macrophage cells.Methods Bacteria from the mucosa tissues of ileum,colon and rectum of inflammatory bowel disease （IBD）patients were isolated,cultured and identified.The toxic genes were confirmed by polymerase chain reaction.The adhesion and invasion ability of bacteria and its survival and proliferation ability in macrophage cells were observed after co-culture with cells.Results Five adherent-invasive Escherichia coli strains were isolated from the intestinal mucosa specimens of two patients with Crohn′s disease and one patient with ulcerative colitis.The adhesion rates＆amp;nbsp;of five bacteria stains （2A,2B,15A,15B,19B）to Caco-2 cells were （1 .4 ± 1 .3 ）%,（1 .5 ± 0.6 ）%, （0.6±0.1）%,（1 .4±0.4）% and （1 .1 ±0.8）%,respectively.The adhesion rates to Int-407 cells were （1 .0±0.8）%,（1 .5 ±0.8）%,（1 .0 ±0.8 ）%,（1 .0 ±0.8 ）% and （0.3 ±0.0 ）%,respectively.The invasion rates to Hep-2 cells were （10.1 ±7.0）%,（0.7 ±0.4）%,（0.4 ±0.3 ）%,（2.2 ±1 .0）% and （2.1 ±1 .8）%,respectively.The invasion rates to Int-407 cells were （0.7 ±0.5 ）%,（0.5 ±0.3 ）%, （2.8±1 .2 ）%,（0.7 ±0.5 ）% and （0.5 ±0.4）%,respectively.All five adherent-invasive Escherichia coli strains could survive and proliferate in macrophage cells.Conclusions Five adherent-invasive Escherichia coli strains are isolated for the first time in Chinese patients with IBD,and all of them could survive and proliferate in macrophage cells.

关 键 词：炎性肠疾病 黏附侵袭性大肠埃希菌 分离 鉴定 

分 类 号：R574[医药卫生—消化系统]