布洛芬对甲醛炎性痛诱导大鼠脊髓神经元Fos蛋白表达的影响  

Effects of Ibuprofen on the Fos Protein Expression in Spinal Cord Neurons of Rats with Inflammatory Pain Induced by Formalin

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作  者:何利平[1,2] 夏东[3] 吴江[4] 

机构地区:[1]武汉大学基础医学院生理学教研室,湖北武汉430071 [2]湖北省通城县人民医院麻醉科,湖北通城437400 [3]武汉大学基础医学院病理学教研室,湖北武汉430071 [4]武汉市妇女儿童医疗保健中心麻醉科,湖北武汉430010

出  处:《武汉大学学报(医学版)》2016年第5期734-737,共4页Medical Journal of Wuhan University

摘  要:目的:观察布洛芬对甲醛刺激大鼠足底引发脊髓神经元Fos蛋白表达的影响,探讨布洛芬对伤害性刺激的抑制作用。方法:SD大鼠30只,随机分为空白对照刺激组(A组),生理盐水注射液组(对照组B组),布洛芬低剂量组(C1组),布洛芬中剂量组(C2组),布洛芬高剂量组(C3组),每组6只。甲醛刺激180min后,用行为学机械触诱发痛阈值测定和免疫组织化学染色法分析大鼠脊髓神经元Fos蛋白表达量及分布状况。结果:30只大鼠均进入结果分析,显示:C1,C2和C3组的机械触诱发痛阈值较对照A组和B组均明显升高(P<0.05),C3组明显高于C1和C2组(P<0.05);而后分析受甲醛刺激后180min时的情况:A组和B组见脊髓背角浅层有大量淡染Fos样免疫阳性神经元,其中A,B均明显多于C1,C2和C3组(P<0.05)。C3组Fos样免疫阳性神经元数明显少于C1和C2组(P<0.05)。结论:布洛芬处理后能对伤害性刺激引起的大鼠机械触诱发痛阈值部分升高,处理能够对脊髓背角浅层Fos样免疫阳性神经元增多效应产生抑制作用;布洛芬可以抑制大鼠Fos样免疫阳性神经元产生,这种表现可能与布洛芬抑制体内前列腺素E合成有关。Objective:To observe the effect of ibuprofen on Fos expression and inflammatory states in rat spinal cord after formalin stimulation. Methods: SD rats were randomly divided into blank control group (A), saline injection control group (group B), low-dose ibuprofen group (C1), middle-dose ibuprofen group (C2 group), and high-dose ihuprofen group (C3 group), with six rats in each group. After formalin stimulation for 180 rain, the rat spinal cord neurons Fos pro- tein expression was detected by immunohistochemical staining, and the mechanical allodynia behavior threshold was determined. Results: Ibuprofen treatment significantly increased the me- chanical allodynia threshold in a dose-dependent manner (P〈0.05). Immunohistochemical re- sults showed that Fos-positive neurons were more in A and B groups than ibuprofen treated groups, and ibuprofen also showed a dose-dependent inhibitory effect. Conclusion: Ibuprofen in- creases noxious stimuli threshold and inhibits Fos-positive neurons maybe through inhibiting prostaglandin E synthesis in vivo.

关 键 词:布洛芬 FOS蛋白 脊髓背角浅层 痛阈值 免疫组织化学染色 

分 类 号:R614[医药卫生—麻醉学]

 

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