青蒿素对小鼠移植乳腺癌MT40作用及CD71表达  

Study of in-vivo Anti-Tumer Effect and Expression of CD71 of Artemisinin on MT40 Cells

作　　者：余和平[1] 崔乐[1] 王必蓉[1] 潘跃进[1] 

机构地区：[1]武汉市普爱医院甲乳外科,湖北武汉430033

出　　处：《武汉大学学报（医学版）》2016年第5期761-764,共4页Medical Journal of Wuhan University

摘　　要：目的:探讨青蒿素对小鼠移植乳腺癌MT40作用及CD71表达。方法:建立MT40荷瘤小鼠模型,分为阴性对照组、青蒿素实验组、阳性对照组,测量各组肿瘤体积、制作生长曲线、检测各组肿瘤组织细胞凋亡率及CD71的表达率。结果:与阴性对照组相比,青蒿素实验组、阳性对照组荷瘤鼠肿瘤生长均受到抑制,青蒿素实验组与阳性对照组比较,t=1.423,P>0.05,青蒿素实验组与阴性对照组比较,t=3.437,P<0.05,有统计学意义;青蒿素实验组细胞凋亡率为21.23%,阳性对照组凋亡率为17.42%,青蒿素实验组与阴性对照组相比,t=8.193,P<0.05,有统计学意义,青蒿素实验组与阳性对照组相比,t=1.140,P>0.05,没有统计学意义;阴性对照组CD71表达阳性率77.81%,阳性对照组CD71表达阳性率70.42%,青蒿素实验组CD71表达阳性率40.23%,青蒿素实验组与阴性对照组相比,t=7.371,P<0.05,有统计学意义,青蒿素实验组与阳性对照组相比,t=5.338,P<0.05,有统计学意义。结论:青蒿素对小鼠移植乳腺癌MT40具有显著抑制生长、诱导细胞凋亡作用,并可以降低肿瘤组织CD71表达,可能通过下调肿瘤组织CD71的表达致抑制肿瘤生长、诱导细胞凋亡作用。Objective：To study the effect of Artemisinin on MT40 cells in-vivo and the change of CD71 expression. Methods： The mouse-borne tumor model of MT40 solid tumor was established to eval- uate the effect of Artemisinin on MT40 cells in-vivo. The tumor bearing mice were divided into 3 groups： group of negative control,group of Artemisinin and group of positive control. We manu- factured the growth curve of every group, and detected the ceils apoptosis by TUNEL and the ex- pression of CDT1 of every group. Results： The tumor growth curve was suppressed both in posi- tive control group and artemisinin group （P〈0.05, versus negative controll P〈0.05, versus positive control）. In apoptosis rate and CD71 expression rate in tumor cells, artemisinin also showed a significant effect similar to positive control （both P（0.05, versus negative control; P〉0.05, versus positive control）. Conclusion： Artemisinin had a strong impact on growth inhibi- tion and apoptosis induction in tumor MT40 cells in-vivo, probably by inhibiting the expression of CD71.

关 键 词：青蒿素 CD71 乳腺癌 

分 类 号：R659[医药卫生—外科学]