端粒酶hTERT反义核酸对TFOF诱导的氟他胺耐受型前列腺癌细胞LNCaP-flu凋亡影响机制研究  

Inhibition of telomerase with hTERT antisense enhances oxytropis falcata-induced apoptosis in flutamide insensitive prostate cancer Cells(LNCaP-flu)

作　　者：高晓东[1] 杨丽霞[1] 李永新[1] 苗海东[1] 

机构地区：[1]甘肃省中医院血液净化中心,兰州730050

出　　处：《中国男科学杂志》2016年第5期14-18,共5页Chinese Journal of Andrology

基　　金：甘肃省中医药管理局科研项目(GZK-2014-67)

摘　　要：目的探讨hTERT基因全硫代修饰反义寡核苷酸(AS PS-ODN)抑制氟他胺耐受型前列腺癌细胞LNCaP-flu端粒酶活性后,藏药镰状棘豆总黄体酮(TFOF)对LNCaP-flu细胞凋亡的影响。方法采用TRAP-PCRELISA法检测氟他胺耐受型前列腺癌细胞LNCaP-flu端粒酶活性;采用台盼蓝观察hTERT AS PS-ODN与TFOF共同作用对氟他胺耐受型前列腺癌细胞LNCaP-flu生长活力的影响;通过双标流式细胞仪测定凋亡细胞的百分率。结果hTERTAS PS-ODN作用于LNCaP-flu细胞48h,端粒酶活性明显下降;hTERTAS PS-ODN作用于LNCaP-flu细胞后加入TFOF 0.1g/L作用48h,LNCaP-flu细胞的抑制率与对照组、S PS-ODN组、AS PS-ODN组、TFOF组及S PS-ODN+TFOF组相比有统计学差异(P<0.01)。hTERTAS PS-ODN作用于LNCaP-flu细胞后加入0.1g/L TFOF作用48h,凋亡细胞的百分率分别与对照组、S PS-ODN组、AS PS-ODN组、TFOF组及S PS-ODN+TFOF组相比有显著性差异(P<0.05)。结论hTER7基因反义核酸对TFOF诱导的氟他胺耐受型前列腺癌细胞LNCaP-flu凋亡有增敏作用。Objective To investigate the effects of telomerase inhibition with hTERT antisense on oxytropis falcata （TFOF）-induced apoptosis in flutamide insensitive prostate cancer cells （LNCaP-flu）. Methods Antisense phosphorothioate oligodeoxynucleotide （AS PS-ODN） was synthesized and purified. Telomerase activity was measured by telomerase PCR ELISA kit. Cell viability was determined by MTT assay;Cell apoptosis was determined by flowcytometry. Results AS PS-ODN significantly inhibited telomerase activity. The cell viability decreased with time after hTERT AS PS-ODN combined with TFOF treatment. The percentage of apoptosis increased with time after hTERT AS PS-ODN combined with TFOF treatment. There was no difference in cell viability and the percentage of apoptosis between S PS-ODN and the control group.. Conclusion Inhibition of telomerase with hTERT antisense can enhance TFOF-induced apoptosis in flutamide insensitive prostate cancer cells （LNCaP-flu）.

关 键 词：端粒 末端转移酶 寡核苷酸类 反义 前列腺肿瘤 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]