利用苹果EST-SSR分析木瓜属种质遗传多样性  

Analysis of Genetic Diversity in Chaenomeles Using Apple EST-SSRs

作　　者：张艳艳[1] 齐红 郭庆梅[1] 孙稚颖[1] 周凤琴[1] 李圣波 

机构地区：[1]山东中医药大学,济南250355 [2]济南市食品药品检验检测中心,济南250001 [3]山东亚特生态技术有限公司,临沂266071

出　　处：《生物技术通报》2016年第7期93-98,共6页Biotechnology Bulletin

基　　金：国家科技支撑计划(2011BAI06B06);山东省科技发展计划项目(2011GSF11904)

摘　　要：利用苹果属的EST-SSR标记对33份木瓜种质资源进行遗传多样性研究,以分析引物在木瓜属中的通用性。筛选出15对引物进行PCR扩增,其中9对引物显示多态性,共扩增出71条带,其中多态性条带59条,多态性条带比率83.10%,并且可成功区分不同种。PIC多态性信息含量平均值为0.45,Nei’s基因多样性(H)、Shannon指数(I)的平均值分别为0.45、0.71。根据EST-SSR数据的UPGMA聚类分析将材料分为五大类,木瓜属的不同基原样本各聚为一支,能很好地将5个种植物区分,显示出单系性。研究结果表明,苹果属的EST-SSR标记在木瓜属上具有高度的可转移性,可应用于木瓜属植物的资源评价及遗传关系研究。EST-SSR markers of Malus pumila Mill.were selected to investigate the genetic diversity of germplasm resources in 33 cultivars of Chaenomeles,and hence to confirm the transferability of SSRs information from Malus to Chaenomeles.In this study,15 selectedprimers were employed for PCR amplification and 9 primers presented polymorphic bands;71 bands were generated by all EST-SSR,of which 59（83.10%）were polymorphic;the different cultivars listed here were successfully distinguished.The average of polymorphic informationcontent（PIC）were 0.45.The average of Shannon index（I） and Nei＇s genetic diversity index（H）were 0.71 and 0.45.UPGMA clusterbased on the EST-SSR data,the materials were divided into 5 groups.Different samples of Chaenomeles were gathered together and could bedistinguished by system tree.The results showed that EST-SSR markers developed from M.pumila showed high transferability to Chaenomeles,and they may be used in germplasm evaluation and genetic relationship for Chaenomeles.

关 键 词：EST-SSR 木瓜属 通用性 

分 类 号：S661.6[农业科学—果树学]