韦兰胶合成菌的原生质体制备与再生研究  

Conditions for Protoplast Preparation and Regeneration by Sphingomonas sp.ATCC 31555

作　　者：周明明[1] 李晓雁[2] 任梦楠 程珂萌 黄海东[1] 

机构地区：[1]天津农学院农学与资源环境学院,天津300384 [2]天津农学院食品科学与生物工程学院,天津300384

出　　处：《生物技术通报》2016年第7期126-130,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31571790)

摘　　要：旨在研究EDTA预处理、菌龄、酶解浓度、酶解温度及酶解时间对Sphingomonas sp.ATCC 31555原生质体制备与再生的影响。结果表明,菌龄10 h,12 mmol/L EDTA预处理,溶菌酶浓度45μg/mL、酶解温度28℃、酶解时间25 min。得到原生质体的形成率达97.3%,再生率达33.9%。研究结果为菌株进行原生质体融合、基因组改组等选育优良菌种提供参考。The effects of EDTA pretreatment,cell age and the concentration,temperature and work time of enzymolysis on protoplastformation and regeneration by Sphingomonas sp.ATCC 31555 were studied.The results showed that the formation rate and regeneration ratereached 97.3% and 33.9% respectively,when the cells cultivated 10 h,pretreated by 12 mmol/L EDTA,using 45 μg/mL of lysozyme,enzymolysis at 28℃ for 25 min.The result of this paper provides a reference for breeding fine microbial strains by protoplast fusion and genomeshuffling.

关 键 词：鞘氨醇单胞菌 韦兰胶 原生质体 再生 

分 类 号：Q93[生物学—微生物学]