羧基张力蛋白在结直肠癌组织中的表达及其临床意义  

Expression and clinical significance of C-terminal tensin-like protein in colorectal cancer

作　　者：陈文琪[1] 李小琴[1] 张晓梅[3] 吴建农[2] 赵小辉[2] 顾汉刚[1] 任琎[1] 王德强[1,4] 

机构地区：[1]江苏大学附属医院肿瘤治疗中心,江苏镇江212001 [2]江苏大学附属医院病理科,江苏镇江212001 [3]南京医科大学附属肿瘤医院遗传与分子生物学研究室,210000 [4]南京医科大学第一附属医院肿瘤科,210029

出　　处：《中华消化外科杂志》2016年第8期809-814,共6页Chinese Journal of Digestive Surgery

基　　金：国家自然科学基金（81301765、81402485）;江苏省自然科学基金（BK20141490）

摘　　要：目的探讨结直肠癌患者中羧基张力（CTEN）蛋白的表达及其与结直肠癌患者临床病理因素和预后的关系。方法采用回顾性病例对照研究方法。收集2010年1月至2012年12月江苏大学附属医院收治153例结直肠癌患者的临床病理资料。采用免疫组织化学染色检测结直肠癌及其癌旁组织中CTEN蛋白的表达。观察指标：（1）免疫组织化学染色检测结直肠癌组织和癌旁组织中CTEN蛋白表达情况。（2）结直肠癌组织中CTEN蛋白表达与结直肠癌患者临床病理因素的关系。（3）结直肠癌组织中CTEN蛋白表达与患者预后的关系。术后采用电话方式进行随访，了解患者生存情况。随访时间截至2015年5月。计数资料采用双侧，检验。采用Kaplan—Meier法绘制生存曲线，Log—rank检验进行生存分析。单因素及多因素COX比例风险模型分析预后因素。结果（1）CTEN蛋白在结直肠癌组织和癌旁组织细胞中主要表达于细胞质，其阳性表达率分别为92．16％（141／153）和20．26％（31／153），两者比较，差异有统计学意义（x^2=160．647，P〈0．05）。35．95％（55／153）的结直肠癌组织和6．54％（10／153）的癌旁组织中CTEN蛋白高表达，两者比较，差异有统计学意义（x^2=39．561，P〈0．05）。（2）肿瘤最大直径〈5cm和≥5cm的结直肠癌组织中CTEN蛋白高表达率分别为16．30％和63．57％，在TNM分期中的Ⅰ～Ⅲ、Ⅳ期中分别为31．15％和54．84％，在无和有神经和（或）脉管侵犯中分别为26．23％和42．39％，上述指标比较，差异均有统计学意义（x^2=38．670，6．026，4．161，P〈0．05）。（3）153例结直肠癌患者中，109例术后接受化疗和（或）放疗和（或）生物靶向治疗。55例CTEN蛋白高表达患者中，接受术后化疗和（或）放疗和（或）生物靶向治疗41例；98例CTEN蛋白低表达者中为68例，两者比较，差异无统计�Objective To investigate the expression of C-temfinal tensin-like （CTEN） protein and its correlations with clinieopathological characteristics and prognosis in patients with coloreetal cancer. Methods The retrospective case-control study was adopted. The clinical data of 153 patients with colorectal cancer who were admitted to the Affiliated Hospital of Jiangsu University between January 2010 and December 2012 were collected. The expression of CTEN protein were detected by immunohistochemiswy. Observed indices included： （1） expression of CTEN protein was detected by immunohistochemistl7. （2） Relationships between the expressions of CTEN protein and clinicopathologica] characteristics of patients. （3） Relationships between the expressions of CTEN protein and prognosis of patients. The follow-up of telephone interview was performed to detect survival of patients untill May 2015. Count data were analyzed using two-sided ehi-square test. Survival curve was drawn using the Kaplan-Meier method, and survival analyses was done using the Log-rank test. Univariate and muhivariate analyses of prognosis were done using COX regression model. Results （ 1 ） Expressions of CTEN protein in cancer tissues and adjacent tissues were located at the cytoplasms. The positive expression rate of CTEN protein was 92.16% （ 141/153 ） and 20.26% （31/153） in cancer tissues and adjacent tissues, respectively, with a statistically significant difference （x^2= 160. 647, P 〈 0. 05）. High expression of CTEN protein was 35.95% （55/153） and 6. 54% （ 10/153 ） in cancer tissues and adjacent tissues, respectively, with a significant diflbrence （x^2 = 39. 561, P 〈 0. 05 ）. （2） The high expression rate of CTEN protein was 16. 30% and 63.57% in tumor with maximum diameter 〈5 cm and ≥5 cm, 31.15% and 54. 84% in the Ⅰ-Ⅲ and Ⅳ stages of TNM stage, 42.39% and 26. 23% in tumor with and without perineural and/or vascular invasion, respectively, showing significant differences （x^2= 38. 670, 6.

关 键 词：结直肠肿瘤 羧基张力蛋白 预后 免疫组织化学染色 

分 类 号：R735.34[医药卫生—肿瘤]