水通道蛋白-1及核因子κB在大鼠重症急性胰腺炎肺损伤中的表达及意义  

Expressions and significance of aquaporin 1 and nuclear factor-kappa B in the severe acute pancreatitis associated lung injury of rats

作　　者：李靖华[1] 张涛[2] 张胜逆[3] 张全[1] 杨季红[1] 程树杰[1] 滑志娟[1] 张彩菊[1] 

机构地区：[1]河北大学附属医院肝胆外科,河北保定071000 [2]河北大学附属医院胃肠外科,河北保定071000 [3]河北大学附属医院皮肤科,河北保定071000

出　　处：《中华消化外科杂志》2016年第8期830-835,共6页Chinese Journal of Digestive Surgery

摘　　要：目的探讨重症急性胰腺炎（SAP）肺损伤大鼠肺组织内水通道蛋白-1（AQP-1）、核因子κB（NF—κB）的表达及意义。方法采用实验研究方法。将48只Wistar大鼠采用随机数字表法分为SAP组和对照组，每组各24只。SAP组大鼠经胰胆管逆行注射5％牛磺胆酸钠（3mL／kg），术后适量补允生理盐水，构建SAP模型。对照组为相同方法注射生理盐水。两组干预后6、12、24、48h分批处死大鼠，每个时柑点6只。（1）采用免疫组织化学染色检测肺组织中AQP—1、NF—κB的表达。（2）采用用T—PCIt检测AQP-1mPLNA的表达。（3）采用DNA断裂的原位末端标记（TUNEL）法检测肺组织细胞凋亡的变化。（4）常规HE染色后显微镜下观察怖组织损伤程度。正态分布的计量资料以x±s表示，组间比较采用方差分析．结果（1）肺组织肉眼观察结果：SAP组大鼠肺组织表现为明显肺水肿，可见肺表面有散在出血点，小川时相点6、12、24、48h肺脏损伤逐渐加重；对照组大鼠肺组织无明显病理学改变。（2）免疫组织化学染色结果显示：SAP组大鼠支气管黏膜上皮细胞、肺泡上皮细胞、浸润的中性粒细胞、单核巨噬细胞中1QP-1、NF-κB表达阳性；对照组大鼠肺组织中只有极少量肺泡间质细胞及肺上皮细胞表达NF—κB。不同时相点（6、12、24、48h）SAP组大鼠肺组织的AQP-1阳性细胞率分别为5．4％±1．7％、6．4％±2．6％、6．3％±1．4％、6．3％±1．6％，对照组分别为28．1％±5．0％、31．2％±5．1％、30．1％±6．2％、29．7％±4．9％，两组比较，差异均有统计学意义（F=27．52，23．89，22．85，22．43，P〈0．05）。SAP组不同时间点大鼠肺组织NF—κB阳性细胞率分别为58．6％±5．0％、77．8％±5．2％、89．3％±4．8％、92．3％±6．8％；对照组分别为5．4％±1．7％、6．4％±2．6％、6．3％±1．4％、6．3％±1．6％，�Objective To investigate expressions and significance of aquaporin 1 （ AQP-1 ） and nuclear factor-kappa B （NF-KB） in lung tissues of rats with severe acute pancreatitis （SAP） associated lung injury. Methods The experimental study was adopted. Forty-eight Wistar rats were divided into the SAP group and control group with 24 rats in each group by random number table. Acute pancreatitis model was induced by retrograde infusion of 5% sodium taurocholate （3 mL/kg） into the biliopancreatic duct and rats were compensated with right amount normal of saline in the SAP group. Rats in the control group were administered with normal of saline using the same method. All the rats were sacrificed at 6, 12, 24 and 48 hours after the operation （n = 6 per group）. （ 1 ） The expressions of AQP-1 and NF-KB in lung tissues were measured using immunohistoehemistry. （2） The expression of AQP-1 mRNA was measured using reahime polymerase chain reaction （RT-PCR）. （3） The apoptosis of cells in lung tissues was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling （TUNEL） method. （4） The degree of lung tissue damage was observed using HE staining. Measurement data with normal distribution were expressed by x ± s, and comparison between groups was done using ANOVA. Results （ 1 ） Visual observation ： lung tissues of rats in the SAP group showed obvious pulmonary edema with scattered bleeding points and lung injury aggravated gradually at 6, 12, 24, 48 hours. Lung tissues of rats in the control group showed no obvious pathological changes. （2） The results of immunohistochemistry showed positive expressions of AQP-1 and NF-KB at bronchial epithelial cells, alveolar epithelial cells, infiltrated neutrophils and mononuclear macrophages in the SAP group, and positive expression of NF-KB at very small amount of alveolar fibroblasts and epithelial cells in the control group. The rate of AQP-1 positive cells in lung tissues of rats at 6, 12, 24, 48 hours were 5.4%±1.7%, 6.

关 键 词：胰腺炎 肺损伤 水通道蛋白-1 核因子-ΚB 细胞凋亡 

分 类 号：R576[医药卫生—消化系统]