电针疗法对围绝经期抑郁症模型大鼠下丘脑-垂体-肾上腺轴及β-内啡肽的影响  

Effects of Electroacupuncture Therapy on Hypothalamic Pituitary Adrenal Axis and β-EP in Rats with Depression

作　　者：蒋希荣 任路[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2016年第8期1923-1925,I0007,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81373718);国家重点基础研究发展计划(973计划)项目(2010CB530401973);辽宁省高等学校优秀人才支持计划项目(LR2014027)

摘　　要：目的:研究电针疗法对围绝经期抑郁症大鼠下丘脑-垂体-肾上腺轴(HPA轴)及β-内啡肽的影响,探究电针疗法对围绝经期抑郁症模型大鼠的作用机制。方法:SPF级SD雌性大鼠60只,随机分为空白组、模型组、假手术组、西药组和电针组,采用双侧卵巢切除和慢性不可预见刺激结合建立围绝经期抑郁症大鼠模型。电针组采用电针刺激"百会"、双侧"肾俞"、双侧"三阴交"。连续治疗28 d,观察大鼠动情周期、糖水偏嗜实验结果,运用酶联免疫吸附法(Elisa法),测定CRH、ACTH、CORT、β-EP的含量。结果:治疗后与模型组相比,电针组及西药组糖水消耗率升高(P<0.05),电针组、西药组及假手术组血清中CRH、ACTH、CORT含量低于模型组(P<0.05);电针组、西药组及假手术组β-EP含量高于模型组(P<0.05)。结论:电针疗法可以通过调节围绝经期抑郁症模型大鼠下丘脑-垂体-肾上腺轴(HPA轴)以及β-EP含量,缓解围绝经期抑郁症症状起防治作用。Objective： To study the effect of electroacupuncture therapy on the hypothalamic pituitary adrenal axis（ HPA axis） and the β-EP in the perimenopausal depression rats and explore the mechanism of electroacupuncture therapy on the model rats. Methods： Sixty SD female rats were randomly divided into groups： blank group,model group,sham operation group,western medicine group and electroacupuncture group. The electroacupuncture group received electroacupuncture at Baihui（ DU20）,Shenshu（ BL23）,bilateral Sanyinjiao（ SP6）. After continuous treatment for 28 days,the estrous cycle and sucrose preference test results were observed. By using enzyme linked immunosorbent assay（ ELISA）,determine the contents of CRH,ACTH,CORT and β-Ep. Results： After treatment,compared with the model group,the electroacupuncture group and western medicine group’s sugar consumption rate increased（ P 〈 0. 05）. The electroacupuncture group,western medicine group and sham operation group’s serum levels of CRH,ACTH and CORT were lower than those in the model group（ P 〈 0. 05）. The electroacupuncture group,western medicine group and sham operation group’s Aβ-EP content was higher than that in the model group（ P 〈 0.05）. Conclusion： The electroacupuncture therapy can regulate the hypothalamus pituitary adrenal axis（ HPA axis） and β-EP content in the model rats and relieve the symptoms of depression.

关 键 词：电针 围绝经期抑郁症 HPA轴 Β-EP 

分 类 号：R-332[医药卫生]