一株耐盐性蛋白酶产生菌的诱变选育及酶学性质研究  

Mutation Breeding and Enzymatic Properties of a Salt-tolerant Protease-producing Strain

作　　者：付瑞敏[1,2] 蔺芳[3] 陈五岭[2] 

机构地区：[1]河南教育学院,郑州450046 [2]西北大学生命科学学院,西安710069 [3]新乡学院生物技术系,河南新乡453003

出　　处：《中国调味品》2016年第8期53-57,64,共6页China Condiment

基　　金：河南省科技攻关重点项目(122102310171);陕西省重大科技创新项目(2009ZKC04-16);河南省高等学校重点科研项目(15B180002);河南教育学院青年科研课题项目(20100103)

摘　　要：通过富集、初筛和复筛从肉联厂附近的土壤中分离筛选耐盐蛋白酶产生菌DB-12,经形态学、生理生化及16SrRNA序列分析,菌株DB-12被鉴定为地衣芽孢杆菌(Bacillus licheniformis),产蛋白酶活力为56U/mL。为增强该菌株的耐盐和产蛋白酶能力,对其进行He-Ne激光诱变,获得遗传稳定性良好的突变株DB-12-16,其所产蛋白酶的酶活最高可达132U/mL,此外,还可耐受25%浓度的氯化钠。酶学性质研究结果表明:突变株所产蛋白酶具有较高的温度和pH适应性。作为一种耐盐且高产蛋白酶的菌,该突变株在食品生产及环境修复等方面有极为广阔的应用前景。A protease-producing strain DB-12 with salt-tolerant ability is isolated from soil and selected out by enrichment culture, prescreening and rescreening. Through morphological, physiological, biological and sequence analysis （16S rRNA）, the strain DB-12 with the protease activity of 56 U/mL is identified as Bacillus licheniformis. In order to enhance its protease-producing and salt-tolerant ability, the strain is mutated by He-Ne laser irradiation. After the mutation, the mutant DB-12-16 with the highest protease-producing ability is selected out and tested on its hereditary stability by analysis of variance. As shown in the result, the protease produced by the mutant DB-12-16 could reach to 132 U/mL. Moreover, compared to the wild strain, the mutant DB-12-16 could endure higher NaCl concentration. Even in the culture with 25% of NaCl concentration, the protease still could maintain its ability. As shown in the result of enzymatic properties, the protease produced by the mutant shows high temperature and pH adaptability. Therefore, as a salt-tolerant and highprotease-producing strain, the mutant DB-12-16 will have a broad application prospect in food production and environment restoration.

关 键 词：耐盐 蛋白酶产生菌 HE-NE激光 诱变选育 

分 类 号：TS201.1[轻工技术与工程—食品科学]