毒死蜱和吡虫啉对意大利工蜂(Apis mellifera L.)的慢性经口毒性  

Chronic Oral Toxicity of Chlorpyrifos and Imidacloprid to Adult Honey Bees( Apis mellifera L. )

作　　者：程燕[1] 谭丽超[1] 卜元卿[1] 葛春男 周军英[1] 单正军[1] 

机构地区：[1]环境保护部南京环境科学研究所,南京210042 [2]南京师范大学,南京210023

出　　处：《生态毒理学报》2016年第2期715-719,共5页Asian Journal of Ecotoxicology

基　　金：2013年环保公益行业项目(2013467026)

摘　　要：文章以毒死蜱和吡虫啉为受试农药,以4日龄内的意大利工蜂(Apis melifera L.)为受试生物,研究2种农药对意大利工蜂的10 d经口慢性毒性。结果显示:参比物质乐果对意大利工蜂的10 d半致死浓度(10 d-LC50)为0.550μg a.i.·g^(-1)食物,平均每天半致死剂量(LDD50)为0.019μg a.i.·蜂^(-1)·天^(-1);在有效试验条件下,毒死蜱对意大利工蜂的10 d LC50为0.582μg a.i.·g^(-1)食物,LDD50为0.021μg a.i.·蜂^(-1)·天^(-1);吡虫啉对意大利工蜂的10 d LC50为0.055μg a.i.·g^(-1)食物,LDD50为1.542 ng a.i.·蜂^(-1)·天^(-1)。试验结果可为毒死蜱和吡虫啉的安全使用提供科学参考,同时可促进我国农药对蜜蜂的安全性评价体系的完善。In the present study, the 10-d chronic oral toxicities of chlorpyrifos and imidacloprid to honeybees（Apis mellifera L.） at age less than 4 days were studied. The validity of the test method was evaluated by testing the chronic toxicity of dimethoate, a reference substance, to the honey bees. For the reference substance, its 10-d LC50 was 0.550 μg a.i.·g^（-1）food and the LDD50 was 0.019 μg a.i.·d^（-1）. These results suggest that the toxicity test method was effective when compared to the ring tests conducted by 17 international laboratories. Under the same experimental conditions, the 10-d LC50 of chlorpyrifos to honeybees was 0.582 μg a.i.·g^（-1）food and the LDD50 was 0.021 μg a.i.·d^（-1）.Correspondingly, the 10-d LC50 of imidacloprid was 0.055 μg a.i.·g^（-1）food and the LDD50 was 1.542 ng a.i.·d^（-1）. Findings of this study are expected to provide scientific basis for the safety assessments of chlorpyrifos and imidacloprid,thus ensuring the safe use of pesticides with respect to honeybee health in China.

关 键 词：乐果 毒死蜱 吡虫啉 意大利工蜂 慢性经口毒性 

分 类 号：X171.5[环境科学与工程—环境科学]