N-3多不饱和脂肪酸的抗炎效应对C57BL/6-PD-1^(-/-)基因敲除小鼠心房电重构的影响  

N-3 polyunsaturated fatty acids prevents atrial electrical remodeling by inhibiting inflammation in C57BL/6-PD-1^(-/-) mice

作　　者：付国强[1] 曹义战[1] 仲月霞[1] 田小溪[1] 王伯良[1] 

机构地区：[1]第四军医大学唐都医院急诊科,西安710038

出　　处：《国际心血管病杂志》2016年第4期235-238,共4页International Journal of Cardiovascular Disease

基　　金：国家自然科学基金(81070161)

摘　　要：目的:观察N-3多不饱和脂肪酸(PUFAs)饲养对C57BL/6-PD-1^(-/-)小鼠体内炎症和心房电重构的影响。方法:观察和比较C57BL/6、PD-1^(-/-)和PUFAs组小鼠的血清炎性细胞因子表达水平和心房有效不应期。结果:与C57BL/6组小鼠相比,PD-1^(-/-)组小鼠血清白细胞介素(IL)-2、IL-4、IL-6、IL-10、IL-17A、肿瘤坏死因子(TNF)和γ干扰素(IFN-γ)水平明显升高,同时PD-1^(-/-)小鼠右心耳、右房低侧壁、右房高侧壁、右房游离壁心房肌有效不应期明显缩短;PUFAs组小鼠血清炎性细胞因子水平较C57BL/6小鼠组大部分仍升高,但升高趋势较PD-1^(-/-)组得到一定程度的遏制,PUFAs组心房有效不应期和C57BL/6组相比无统计学差异。与PD-1^(-/-)组相比,PUFAs组血清炎性细胞因子水平均明显降低,心房有效不应期明显延长。结论:PD-1基因敲除后C57BL/6小鼠体内炎性细胞因子水平明显升高,并出现心房有效不应期缩短,N-3多不饱和脂肪酸饲养能够遏制这种变化趋势。Objective： To investigate the effect of dietary N-3 polyunsaturated fatty acids on the inflammation and atrial electrical remodeling in C57BL/6-PD-1-/- mice. Methods： The expression of inflammatory eytokines and the atrial effective refractory periods （AERPs） in C57BL/6, PD-1 / and PUFA groups were observed and compared. Results. The interleuldn （IL）-2, IL-4, IL-6, IL-10, IL-17A, tumor necrosis factor（TNF） and γ-interferon （IFN-γ） were significantly increased in PD-1-/ group compared with C57BL/6 group; meanwhile, the PD-1 -/- micegroup appeared significantlyshorter AERPs in the right atrial appendage, low lateral wall, high lateral wall and free wall of right atrium compared with C57BL/6 group. Most of the inflammatory cytokines in PUFAs group mice still rose compared with C57BL/6 mice, but the rising trend had a certain degree of containment compared with PD-1-/- mice; at the same time the AERPs in PUFAs mice had no statistical difference compared with C57BL/6 mic; The PUFA group demonstrated asignifieantly lower inflanmmtory cytokine level and longer AERPs compared with PD-1 /- group. Conclusion.. Our findings strongly support that the inflammatory cytokines levels increase significantly in PD-1-/ mice, at the same time PD-1-/- mice appear significantly shorter AERPs, dietary anti-inflammation drug N-3 PUFA supplementation can curb this trend.

关 键 词：PD-1基因敲除 炎性细胞因子 心房电重构 心房颤动 N-3多不饱和脂肪酸 

分 类 号：R541.75[医药卫生—心血管疾病]