激活素A在食管癌组织中的表达水平及与肿瘤分化和转移的关系  

The expression levels of activin A in esophageal cancer and its relationship with tumor differentiation and metastasis

作　　者：王永连[1] 刘尚国[1] 陶义鹏[1] 王毅[1] 王忠民[1] 李汉臣[1] 

机构地区：[1]新乡医学院第一附属医院胸外科,河南新乡453100

出　　处：《重庆医学》2016年第22期3043-3045,3048,共4页Chongqing medicine

基　　金：河南省卫生厅立项课题(200803080)

摘　　要：目的探讨激活素A在食管癌组织中的表达水平变化,以及与肿瘤分化和转移之间的关系。方法选取该院2010年6月到2012年6月收治的57例食管癌患者作为研究对象,同时选取因食管不适来院检查确诊无病变的36例患者作为对照研究。分别采用实时荧光定量PCR和免疫组织化学分析食管癌组织和正常食管组织激活素A mRNA和蛋白的表达水平,并分析激活素A与食管癌患者肿瘤分化、转移和术后生存复发之间的关系。结果食管癌组织中激活素A mRNA和蛋白的表达水平明显高于正常食管组织(P<0.05)。低分化和中分化食管癌组织中激活素A表达水平明显高于正常食管组织和高分化食管癌组织(P<0.05),低分化食管癌组织中激活素A水平明显高于中分化(P<0.05)。淋巴结转移患者中激活素A水平明显高于未转移患者(P<0.05)。激活素A阳性患者术后1、2、3年累积生存率明显低于激活素A阴性患者(P<0.05),而累积复发率明显高于激活素A阴性患者(P<0.05)。结论食管癌组织中激活素A表达异常,与肿瘤分化、转移和术后生存和复发密切相关,可作为临床预后辅助诊断指标。Objective To investigate the changes of activin A expression levels in esophageal carcinoma and its relationship with tumor differentiation and metastasis. Methods Totally 57 cases of esophageal cancer patients from June 2010 to June 2012 in our hospital were selected as research subjects, while 36 patients that esophageal discomfort and diagnosed without lesions were selected as a control study. Activin A mRNA and protein expression level were analyzed by real-time PCR and immunohistochemistry. The relationship between activin A and tumor differentiation,metastasis, survival and recurrence were analyzed. Results The expression level of activin A mRNA and protein th in observation group significantly enhanced than that of normal esophageal tissues（P〈0.05）. The activin A levels in poorly and moderately differentiated esophageal tisgue were significant higher than that of well-differentiated（P〈0.05）. The activin A levels in poorly differentiated esophageal tissue expression of were significant higher than that of and moderately differentiated esophageal tissue（P〈0.05）. There was positive correlation between differentiation degree and activin A expression levels. The activin A levels in patients with lymph node metastasis were were significantly higher than those without metastasis（P〈0.05）. 1,2 and 3 year cumulative survival rate in high expression of activin patients was significantly lower than that of low expression of activin A（P〈0.05）. 1,2 and 3 year cumulative recurrence rate in high expression of activin patients was significantly higher than that of low expression of activin A（P〈0.05）. Conclusion The activin A in esophageal cancer tissue exhibit abnormal level. There are closely relationship between activin A and tumor differentiation,metastasis and postoperative survival and recurrence rates,can be used as a diagnostic marker of clinical outcomes.

关 键 词：食管肿瘤 肿瘤分化 肿瘤转移 生存和复发 激活素A 

分 类 号：R735.1[医药卫生—肿瘤]