微生物发酵对MFC产电的影响  

Effect of microbial fermentation on electricity production of microbial fuel cells

作　　者：李晶[1] 袁林江[1] 刘正川[1] 周国标[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《环境工程学报》2016年第8期4049-4054,共6页Chinese Journal of Environmental Engineering

基　　金：国家水体污染控制与治理科技重大专项(2009ZX07212-002)

摘　　要：采用2瓶型微生物燃料电池(MFC),以葡萄糖为基质,对MFC内发酵产物和参与的细菌进行了分析,探讨了微生物发酵与产电之间的关系。结果表明,以葡萄糖为基质产电的过程中,葡萄糖被发酵成乙酸、丙酸、乳酸3种主要产物。乙酸和丙酸都可以直接被微生物利用产电,乙酸更易被产电微生物利用,且能产出较高电压的电;乳酸需经过进一步发酵转化为丁酸、丙酸和乙酸,丁酸经进一步代谢为乙酸和丙酸后才能被用于产电。在MFC发酵产电中后期,阳极表面生物膜里紧密聚集着大量杆菌,主要有Klebsiella sp.、Citrobacter sp.、Lactococcus sp.、Pantoea sp.以及Proteobacterium近缘菌,而在阳极室污泥中出现的优势菌为Citrobacter sp.、Clostridiales目的细菌、Eubacterium limosum近缘菌。因此,认为MFC产电是由发酵菌联合产电菌通过梯次降解基质、分步利用、协同代谢的结果。The relationship between microbial fermentation and electricity production was explored by analyzing the bacterium and fermentation products using two microbial fuel cell（ MFC） bottles with glucose as the substrate. The results indicate that glucose is mainly fermented to three kinds of carboxylic acids in the MFC：acetic acid,propionic acid,and lactic acid. Acetic acid and propionic acid can be used directly to produce electricity,where acetic acid is more easily oxidized by electricigens to generate a higher voltage. Lactic acid must first be decomposed to butyrate acid,propionic acid,and acetic acid,after which butyrate acid is decomposed to a mixture of metabolites,including acetic acid and propionic acid; these products are then used to produce electricity. The surface of an anode was coated by a large number of bacilli in the form of a biofilm that mainly comprised Klebsiella sp.,Citrobacter sp.,Lactococcus sp.,Pantoea sp.,and Proteobacterium. The predominant bacteria in the anode chamber sludge were Citrobacter sp.,Clostridiales,and Eubacterium limosum. It is proposed that the electricity generation in the MFC results from cooperative metabolism of electricigens and fermentation bacteria via substrate decomposition and step-by-step utilization of products.

关 键 词：MFC 发酵 产电微生物 

分 类 号：X703[环境科学与工程—环境工程]