三株拮抗菌对导致马铃薯晚疫病的致病疫霉的抑制作用比较  

Comparison of Three Antagonistic Bacteria against Phytophthora infestans in Potato

作　　者：张亚辉[1] 蒋继志[1] 王蓓蓓[1] 李颖[1] 万安琪 沙海天[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《作物杂志》2016年第4期162-166,共5页Crops

基　　金：河北省自然科学基金(C2015201231);河北省科技支撑计划项目(13226502D)

摘　　要：为了明确前期获得的对致病疫霉有显著抑制和诱抗作用的拮抗菌中生防潜力更大的菌株,以对峙培养法、打孔法、离体组织切片法对菌株Sy11、M15和A5295抑制致病疫霉的部分性能和抑菌效果进行了比较。结果显示,在抑制致病疫霉菌丝生长和孢子萌发方面,Sy11和M15均显著优于A5295,在马铃薯块茎切片上预防晚疫病方面,三者之间无显著差异;但Sy11在抑制致病疫霉孢子囊形成和游动孢子萌发方面则明显优于M15;Sy11的抑制率分别为68.9%和71.3%,M15的抑制率分别为56.7%和67.3%,两者之间有显著差异。表明Sy11菌株对马铃薯晚疫病的防治具有更大的应用潜力。Sy11, M15 and A5295 are antagonistic strains of Phytophthora infestans, screened in previously experiments in our lab. In order to clear which is the most potential strain, the partial inhibitory characteristics of the three strains on P.infestans against and control efficiency of late blight on potato tuber slices were compared with dual-culture, punch method, and potato tuber slices, respectively. The results showed that both Sy11 and M15 were evident better than A5295 in suppression mycelial growth of P. infestans and spore germination, and three strains had no significant differences in control late blight on potato tuber slices. But Sy11 was stronger than M15 in restraining sporangia formation and zoospores germination, the inhibition rates of former were 68.9% and 71.3%, respectively, that of later were 56.7% and 67.3%, respectively, there were significant differences between Sy11 and M15. These findings showed that Sy11 strain might have the most potential in control potato late blight in future.

关 键 词：致病疫霉 马铃薯 拮抗菌 抑菌作用 离体组织防病 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]