康艾注射液体外对结肠癌 Lovo 细胞凋亡和增殖的影响  

Effects of KangAi injection on apoptosis and proliferation of rectal cancer Lovo cells in vitro

作　　者：张腾腾[1] 杨振[2] 

机构地区：[1]商丘市第一人民医院肿瘤科,河南省商丘476000 [2]郑州大学第一附属医院胃肠外科,河南省郑州450052

出　　处：《中国基层医药》2016年第16期2425-2428,共4页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的：观察康艾注射液在体外对结肠癌 Lovo 细胞凋亡和增殖的影响。方法采用 Sub-G1法、Annexin-V ／PI 法检测康艾注射液体外对结肠癌 Lovo 细胞凋亡的影响；Real-Time PCR（荧光定量 PCR）法检测康艾注射液体外作用结肠癌 Lovo 细胞后 Caspase-3、Bcl-2在 mRNA 表达水平的变化；流式细胞仪法检测康艾注射液体外对结肠癌 Lovo 细胞作用后增殖蛋白 Ki-67的表达。结果康艾注射液180μL／mL 处理 Lovo 细胞48 h 后，凋亡细胞比例 Sub-G1法对照组为（4．75±1．04）％，试验组为（45．61±5．22）％（t ＝7．68，P ＜0．001），Annexin-V ／PI 法对照组凋亡细胞比例为（7．28±1．99）％，试验组为57．02±3．88）％，两组差异有统计学意义（t ＝11．42，P ＜0．01）；Caspase-3表达增加（t ＝4．06，P ＜0．05），Bcl-2表达降低（t ＝5．69，P ＜0．01），两组差异有统计学意义；增殖蛋白 Ki-67对照组阳性表达率为（95．79±1．66）％，试验组为（65．84±4．80）％，两组差异有统计学意义（t ＝5．90，P ＜0．01）。结论康艾注射液在体外能够有效促进结肠癌 Lovo 细胞的凋亡，抑制其增殖。Objective To investigate the effects of KangAi injection on apoptosis and proliferation of rectal cancer Lovo cells in vitro.Methods SubG1 and Annexin -V /PI were used to examine the apoptosis of Lovo cells in vitro.Real -time PCR was used to examine the expression of Caspase -3 and Bcl -2 at mRNA level.Proliferation protein ki -67 was detected by flow cytometry.Results Lovo cells treated with KangAi injection 180μL/mL for 48h increased apoptosis rate from （4.75 ±1.04）% to （45.61 ±5.22）% with SubG1 （t =7.68,P 〈0.01 ）,and （7.28 ±1.99）% to （57.02 ±3.88）% with Annexin -V /PI（t =11.42,P 〈0.01）.Lovo cells treated with KangAi injection 180μL/mL for 48h increased the expression of Caspase -3 at mRNA level（t =4.06,P 〈0.05）,while Bcl-2 decreased（t =5.69,P 〈0.01 ）.Lovo cells treated with KangAi injection 180μL/mL for 48h decreased the expression of ki -67 from （95.79 ±1.66）% to （65.84 ±4.80）%（t =5.90,P 〈0.01 ）.Conclusion KangAi injection can promote apoptosis and inhibit proliferation of rectal cancer Lovo cells in vitro.

关 键 词：结肠肿瘤 细胞凋亡 细胞增殖 康艾注射液 

分 类 号：R735.35[医药卫生—肿瘤]