棘腹蛙“水肿病”病原的分离鉴定  

Isolation and Identification of Pathogenic Bacteria Caused “Edema Disease” in Paa boulengeri

作　　者：李晓英[1] 罗洁[1] 杜孝田[1] 龙方伟 胡凯[1] 徐敬明[1] 樊汶樵[1] 

机构地区：[1]重庆文理学院林学与生命科学学院,重庆珍稀濒危水产资源保护与开发研究中心,重庆402168

出　　处：《动物医学进展》2016年第8期116-119,共4页Progress In Veterinary Medicine

基　　金：重庆市基础与前沿技术研究(重点)专项(cstc2015jcyjBX0013);重庆市基础与前沿研究计划一般项目(cstc2014jcyjA80042);重庆文理学院人才引进项目(R2013LS13,R2014LX07)

摘　　要：为了解重庆地区部分棘腹蛙养殖场"水肿病"病因,并对该病的临床防控提供参考,本研究采集了10只具有"水肿病"典型症状的2岁龄棘腹蛙成蛙,剖检后从其主要病变组织分离细菌;对分离的优势菌进行培养特性观察和回归致病性试验,同时进行生理生化特性鉴定、16SrDNA序列测定和药敏试验。结果显示,从病蛙腹腔中分离到1株优势菌株并命名为CQWU201501,该菌为革兰阴性无芽胞短杆菌,主要生理生化特性符合弗氏柠檬酸杆菌的指标,16SrDNA序列与弗氏柠檬酸杆菌同源性高达99.5%;药敏试验显示该菌对绝大部分β-内酰胺类、氨基糖苷类、喹诺酮类和四环素类药物敏感。综上所述,棘腹蛙"水肿病"是由弗氏柠檬酸杆菌引起的;该菌对供试的16种药物敏感,仅对头孢唑啉和复方新诺明2种药物耐药,生产上可选择敏感药物治疗。To understand the main biological characteristics of pathogen in Paa boulengeri that suffered from ＂edema disease＂ and the methods to treat this disease, 10 adult frogs （2 years old） with the typical symptoms of edema disease were collected, and candidate bacteria were isolated from the pathological tissue after dissection.Then the cultured characteristics observation, pathogenic experiment, physiological and biochemical identification, 16 S rDNA sequence, and drug sensitivity test of candidate bacteria were conducted. Results showed that a Gram negative and nonspore bacillus, named as CQWU201501, was isolated from the peritoneal cavity. Physiological and biochemical characteristics and 16 S rDNA sequence indicated as Citrobacter freundii.Drug sensitivity test revealed that the strain was sensitive to the majority drug of β- lactam, aminoglycosides, quinolones and tetracycline.Above all, the Citrobacter freundii strain was bacterial pathogen leading to ＂edema disease＂ of Paa boulengeri; this pathogeny was sensitive to 16 drugs,only resistant to cefamedin and compound sulfamethoxazole.Appropriate drugs could be chosen for symptomatic treatment.

关 键 词：棘腹蛙 水肿病 分离鉴定 药敏试验 

分 类 号：S917.1[农业科学—水产科学]