人脐带间充质干细胞抑制卵巢癌的初步研究  

Effect of Human Umbilical Cord-derived Mesenchymal Stem Cells on The Growth of Ovarian Cancer Cells

作　　者：向江东[1] 周莉娜[1] 李林霞[1] 席晓薇[1] 陈威[1] 

机构地区：[1]上海交通大学附属第一人民医院妇产科,上海200080

出　　处：《现代生物医学进展》2016年第23期4406-4409,4430,共5页Progress in Modern Biomedicine

基　　金：上海市自然科学基金项目(13ZR1459600)

摘　　要：目的:从足月剖腹产分娩新生儿脐带中分离出人脐带间充质干细胞(UC-MSCs),探讨其在体外促进SKOV3卵巢癌细胞凋亡,抑制其增殖的作用。方法:新鲜人脐带洗净后剥离动静脉及脐带外膜,得到脐带Wharton's胶。采用组织块贴壁法分离、纯化得到UC-MSCs细胞,光镜下观察UC-MSCs细胞的形态及贴壁生长情况。收集UC-MSCs细胞培养上清,加入SKOV3细胞共培养后,观察不同作用时间(12 h,24 h,36 h,48 h,60 h,72 h)其体外促进SKOV3卵巢癌细胞凋亡,抑制其增殖的作用。结果:光镜下UC-MSCs细胞成长梭状,单核,并成放射或漩涡状排列。PI染色提示,随着UC-MSCs细胞培养上清对SKOV3卵巢癌细胞作用时间的增加,其发生凋亡的细胞数量增多,且具有统计学意义(P<0.05)。MTT实验提示SKOV3细胞增殖活力随UC-MSCs细胞培养上清作用时间的增加而显著下降(P<0.05),共培养24 h,48 h,72 h的抑制率分别为17.08%,35.36%,46.83%。结论:UC-MSCs在体外具有明显促进SKOV3卵巢癌细胞凋亡,抑制其增殖的作用。Objective： The aim of this study is to isolate human umbilical cord-derived mesenchymal stem cells from newborn human umbilical cord and explore the effect of UC-MSCs on the proliferation, as well as apoptosis of SKOV3 cells in vitro. Methods：The fresh human umbilical cord tissues were obtained and washed under sterile conditions. Arteries and vein were removed in order to get Wharton’s jelly. UC-MSCs were isolated and amplified via tissue-cultivation method. Morphology and adherent condition of UC-MSCs were observed under the optical microscope. UC-MSCs cell culture supernatant was collected, and then co-cultured with SKOV3 cells. According to co-culture time, we divided the study group into 6 groups： 12 h, 24 h, 36 h, 48 h, 60 h and 72 h. We evaluated the activities of UC-MSCs in apoptosis and proliferation of SKOV3 cells. Results： Fibroblast-like cells were successfully isolated from human umbilical cord tissues using the direct tissue-cultivation method. The UC-MSCs cells formed parallel or whirlpool-like arrays when a confluent monolayer had developed. PI stain test showed UC-MSCs could dramatically induce apoptosis of SKOV3 cells（P〈0.05）. MTT test revealed UC-MSCs could significantly inhibit proliferation of SKOV3 cells（P〈0.05）. Conclusions：UC-MSCs can be successfully isolated from newborn human umbilical cord tissue by tissue-cultivation method. UC-MSCs can induce apoptosis and inhibit proliferation of SKOV3 cells in vitro.

关 键 词：脐带间充质干细胞 卵巢癌 细胞凋亡 细胞增殖 

分 类 号：R737.31[医药卫生—肿瘤]