BK通道对大鼠肾脏缺血再灌注损伤的作用及机制  

The Role of BK Channel in Rat Renal Ischemia Reperfusion Injury and Related Mechanisms

作　　者：谭超[1,2] 赵晓光[3] 袁建林[1] 刘贺亮[1] 韩超[1] 张昆[1] 张强 厐卫东 贺彦平 刘飞[1] 

机构地区：[1]第四军医大学西京医院泌尿外科,陕西西安710032 [2]西安141医院外一科,陕西西安710089 [3]解放军第413医院外一科,浙江舟山316004

出　　处：《现代生物医学进展》2016年第23期4410-4414,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81270844)

摘　　要：目的:探讨缺血再灌注损伤早期肾脏皮质内大电导钙依赖性钾通道(BK)通道的表达及意义。方法:建立成年SD大鼠肾脏急性缺血再灌注损伤模型,快速收集24小时缺血大鼠与对照大鼠血和损伤侧肾脏皮质标本,使用ELISA方法检测血肌酐和尿素氮含量,实时荧光定量RT-PCR和蛋白免疫印迹方法检测肾脏组织中BK通道α亚基的m RNA表达水平和蛋白表达水平。结果:(1)急性缺血再灌注大鼠损伤侧肾脏皮质BK通道α亚基m RNA水平较对照大鼠同侧肾脏皮质的表达明显降低(P<0.01)。(2)急性缺血再灌注大鼠损伤侧肾脏皮质BK通道α亚基蛋白水平较对照大鼠同侧肾脏皮质的表达也明显降低(P<0.05)。(3)NS1619预处理缺血再灌注大鼠血尿素氮和血肌酐含量显著降低(P<0.05)。结论:BK通道表达和功能的改变是参与大鼠肾脏缺血再灌注损伤的重要机制。Objective： To study the expression and effects of BK channel in the cortex of kidney after ischemia reperfusion injury.Methods： The model of Ischemia reperfusion in the kidney was developed in SD rats. The blood and the kidney tissues were collected at 24 h after IR. The values of BUM and Scr were tested by the ELISA, and the m RNA and protein expression of BK channelα subunit were examined by the RT-PCR and western blot. Results： The m RNA expression of BK channel α subunit in IR group rats were were significantly decreased compared with that in sham group rats（P〈0.01）. The protein expression level of BK channel α subunit in IR group rats were significantly lower than that in sham group rats（P〈0.05）. The values of BUM and Scr in IR rats after NS1619 treatment was significantly decreased than the IR rats with saline. Conclusion： The expression and function of BK channel play an important rolein rat renal ischemia reperfusion injury.

关 键 词：缺血再灌注 肾脏 大电导钙依赖性钾通道 Α亚基 

分 类 号：R-33[医药卫生] R692