MAGEA在骨肉瘤中的表达和预后分析  

The expression level of MAGEA in osteosarcoma and its association with prognosis

作　　者：邹昌业[1] 徐韶[2] 唐清连[1] 杨峥[3] 尹军强[1] 谢显彪[1] 黄纲[1] 王晋[1] 沈靖南[1] 

机构地区：[1]中山大学附属第一医院骨肿瘤科,广州市510080 [2]南方医科大学第三附属医院口腔科,广州市510630 [3]中山大学附属第一医院病理科,广州市510080

出　　处：《实用医学杂志》2016年第16期2636-2641,共6页The Journal of Practical Medicine

基　　金：国家自然科学基金青年项目(编号:81001194);教育部高等院校博士学科点专项基金(编号:20100171120077)

摘　　要：目的:研究恶性黑色素瘤抗原基因A(MAGEA)在骨肉瘤中的表达特点及与骨肉瘤预后的相关性。方法:利用基因芯片方法筛选在骨肉瘤中特异性高表达基因。利用Realtime PCR和Western-blot的方法验证其在骨肉瘤细胞和组织中的表达。用免疫组化方法研究其在骨肉瘤组织中的表达,分析其与预后的关系。结果:MAGEA家族基因在骨肉瘤中协同高表达,在正常成骨细胞中无表达。MAGEA在肺转移灶中的表达水平明显高于原发灶。MAGEA表达阳性的骨肉瘤患者肺转移风险升高(RR=2.79,95%CI,1.12-6.93,P=0.028),预后较差(80%±8.9%vs 39.6%±8.4%,P=0.01)结论 :MAGEA家族基因在骨肉瘤组织中的高表达,是骨肉瘤预后的重要标志。Objective To investigate the expression level and clinical significance of melanoma antigen gene A（MAGEA） in osteosarcoma patients. Methods Compare gene expression profiles in osteosarcoma cell lines and osteoblasts with gene microarrays. Validation of differentially expressed genes was carried out by realtime polymerase chain reaction analysis. Corresponding protein levels were measures by Western blot analysis in osteosarcoma cell lines and by immunohistochemistry in osteosarcoma tissues. The staining intensity of immunohistochemistry was correlated with clinical outcome, and its prognostic significance was analyzed. Results Several genes belonging to MAGEA increased significantly in all osteosarcoma cell lines and tumor tissue, but not in normal osteoblast cell. Patients with MAGEA expression has higher risk of lung metastasis（relative risk 2.79,95% confidence interval, 1.12-6.93; P = 0.028） and lower five-year survival rates（39.6% ± 8.4% vs. 80% ±8.9%, P = 0.01） compared with patients without MAGEA expression. Conclusions The expression of MAGEA increased in osteosarcoma, which inversely correlating with outcome of osteosarcoma patients.

关 键 词：骨肉瘤 基因芯片 肿瘤睾丸抗原基因 预后 

分 类 号：R738.1[医药卫生—肿瘤]