TG2过表达对人乳腺癌MCF-7细胞阿霉素耐药性的影响  

EFFECT OF TG2 OVER-EXPRESSION ON DRUG RESISTANCE TO ADRIAMYCIN IN HUMAN BREAST CANCER MCF-7 CELLS

作　　者：李健林[1] 贺文兴[1] 全军利 吴斯敏[1] 王桂芬[1] 刘志明[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,南宁530021

出　　处：《广西医科大学学报》2016年第4期583-587,共5页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助项目(No.2015GXNSFAA139225)

摘　　要：目的:探讨Ⅱ型谷氨酰胺转氨酶(TG2)过表达对人乳腺癌MCF-7细胞阿霉素耐药性的影响。方法:将MCF-7细胞分为3组:转染过表达TG2组(TG2组)、空白对照组(CON组)、阴性对照组(NC组)。采用荧光定量PCR(FQ-PCR)法和蛋白免疫印迹(western blot)法分别检测各组细胞TG2mRNA和蛋白表达,流式细胞术检测细胞凋亡情况,MTT法测定阿霉素对各组细胞处理后的半数抑制浓度(IC50)。结果:TG2组TG2mRNA相对表达量和蛋白的表达量较CON组、NC组显著升高,TG2组细胞凋亡率较CON组、NC组显著降低,TG2组IC50较CON组、NC组显著升高,差异均具有统计学意义(均P<0.05)。结论:通过慢病毒转染成功使人乳腺癌MCF-7细胞TG2过表达,且TG2的过表达能增加人乳腺癌MCF-7细胞对阿霉素的耐药性,提示TG2有可能成为治疗肿瘤耐药的一个重要靶点。Objective:To investigate the effect of typeⅡ transglutaminase(TG2)over-expression on drug resistance to adriamycin in human breast cancer MCF-7cells.Methods:MCF-7cells were divided into 3groups:the TG2 group in which MCF-7cells were infected by lentivirus vectors carrying TG2 gene,the blank control(CON)group and the negative control(NC)group.The mRNA and protein expression of TG2 were determined by fluorescent quantitation polymerase chain reaction(FQ-PCR)and western blotting,respectively.Cell apoptosis was detected by flow cytometry.Median inhibitory concentrations(IC50)of MCF-7treated with adriamycin at different dosage were evaluated with MTT assay.Results:The mRNA and protein expression of TG2 in TG2group were higher than those in the CON and the NC groups(P <0.05).The apoptosis rate of TG2over-expressed-MCF-7cells was significantly lower than that in the CON and the NC groups(P <0.05).Moreover,the IC50 of adriamycin to MCF-7in TG2 group was significantly increased,compared with the CON and the NC groups(P <0.05).Conclusion:Over-expression of TG2 could increase the sensitivity of human breast cancer MCF-7cells to adriamycin,and TG2 can be a potential anti-cancer target.

关 键 词：Ⅱ型谷氨酰胺转氨酶 乳腺癌 耐药 阿霉素 

分 类 号：R737.9[医药卫生—肿瘤]