神经节苷脂对脂多糖损伤大鼠嗜铬细胞瘤细胞的保护作用  

The protective effects of gangliosides on LPS-induced PC12 cells injury

作　　者：宋英[1] 许西西 丁维[1] 茅东升[1] 

机构地区：[1]浙江工业大学药学院,杭州310014

出　　处：《中国应用生理学杂志》2016年第4期310-313,I0011,共5页Chinese Journal of Applied Physiology

基　　金：浙江省自然基金(LY13H090006);全国临床药学研究基金(L2011079)

摘　　要：目的:研究内源性神经节苷脂对大鼠嗜铬细胞瘤细胞株(PC12)脂多糖(LPS)损伤后的作用及机制。方法:培养PC12细胞,建立LPS损伤模型,采用MTT法检测不同浓度LPS对PC12细胞存活率的改变、葡萄糖神经酰胺合成酶抑制剂D(D-PDMP)耗竭内源性神经节苷脂时LPS对PC12细胞存活率的改变以及添加外源性神经节苷脂GM1后对PC12细胞存活率的保护作用;倒置显微镜和荧光显微镜观察细胞状态;RT-PCR检测NF-κB的相对表达含量。结果:LPS能导致PC12细胞形态学的改变及存活率下降,且随着LPS浓度的增加细胞存活率逐渐降低;神经节苷脂GM1能明显改善LPS所致的细胞形态学以及存活率的改变;工具药D-PDMP耗竭内源性神经节苷脂后,LPS对PC12细胞的损伤更严重,添加外源性神经节苷脂GM1,细胞形态学及存活率得到明显改善,细胞存活率上升;LPS损伤时细胞内NF-κB含量增加;D-PDMP预先耗竭内源性神经节苷脂时NF-κB含量增加更多;添加外源性神经节苷脂GM1后NF-κB含量降低。结论:内源性神经节苷脂对PC12细胞LPS损伤具有保护作用,可能是通过NF-κB信号通路来发挥作用的。Objective： To investigate the protective ettects of endogenous gangliosides on 12S-induced PC12 cells injury and its possible mechanism. Methods： PC12 cells were cultured, and lipopolysaceharide（LPS） injury model was established. We detected the changes in survival rate of different eoncentrations of I,PS on PC12 cells, and the changes in survival rate of LPS when endogenous gangliosides were exhausted by D-threo-1-phenyl-2-deeanoylamino-3-morpholino-l-propanol（D-PDMP）, and the protective effects of monosialoganglioside （GMI） on LPS-induced PC12 cells injury. Meanwhile, we ohserved the morphological changes of GM1 on PC12 cells induced after LPS injury hy inverted microseope and fluorescence microscope, and then we detected the relative expression of NF-κB. Results： LPS could decrease the survival rate of cells in a concentration-dependent manner, and GM1 could significantly protect the cells against the changes in survival rate and morphological damages induced by LPS; After depletion of endogenous gangliosides by D-PDMP, LPS had more injury to PC12 cells, which could be improved by adding GM1 ; RT-PCR results showed that the relative expression of NF-κB in PC12 cells was increased in LPS group, while relative expression of NF-κB increased mueh higher when endogenous gangliosides were exhausted by D-PDMP, and the relative expression of NF-κB was decreased after GM1 being added. Conclusion： The endogenous gangliosides might through NF-κB signal pathway to protect PC12 cells against IPS-induced injury.

关 键 词：神经节苷脂GM1 大鼠嗜铬细胞瘤细胞株 脂多糖 NF-ΚB 

分 类 号：R741[医药卫生—神经病学与精神病学]