结直肠腺瘤患者结肠黏膜组织环氧酶-2的表达研究  

Study on COX-2 Expression in Colonic Mucosa of Patients with Colorectal Adenoma

作　　者：杨静[1] 朱元民[2] 吴杰[1] 张黎明[2] 刘玉兰[2] 

机构地区：[1]武汉市中心医院消化内科,湖北武汉430014 [2]北京大学人民医院消化内科

出　　处：《华南国防医学杂志》2016年第7期440-445,共6页Military Medical Journal of South China

摘　　要：目的探讨结直肠腺瘤(colorectal adenoma,CRA)患者一般临床特征、腺瘤不同发展阶段和伴发代谢综合征(metabolic syndrome,MetS)因素情况与结肠黏膜组织环氧酶-2(cyclooxygenase-2,COX-2)表达水平的关系。方法收集51例CRA患者(CRA组)和8例无CRA患者(对照组,不伴发任何MetS因素)左半结肠外观正常黏膜组织。采用免疫组织化学方法检测COX-2表达水平,计算黏膜腺上皮COX-2着色评分、腺上皮COX-2表达阳性率和固有层COX-2阳性细胞数,分析上述指标与CRA患者性别、年龄、吸烟史、饮酒史、胆囊切除史、消化道肿瘤史和结直肠肿瘤家族史等一般临床特征的关系;按不同阶段CRA、患者是否伴发MetS因素分别对CRA患者进行分组,各组进行对比分析。结果 CRA患者一般临床特征与COX-2表达水平无统计学意义(P>0.05)。进展性腺瘤患者固有层COX-2阳性细胞数明显高于对照组,组间比较差异具有统计学意义(P<0.05)。伴发MetS因素患者COX-2腺上皮着色评分和腺上皮COX-2表达阳性率均明显高于不伴发MetS因素患者,组间比较差异具有统计学意义(P<0.05)。伴发MetS因素组固有层COX-2阳性细胞数均值明显高于不伴发MetS因素组(P<0.01)和对照组(P<0.01)。结论 CRA患者结肠黏膜组织COX-2表达水平与患者伴发MetS因素密切相关。Objective To investigate the relationship between general clinical characteristics,different stages of adenomas,metabolic syndrome（MetS）factors of patients with colorectal adenoma（CRA）and cyclooxygenase-2（COX-2）expression in colonic mucosa.Methods Samples of normal mucosa in left colon from 51 CRA patients（CRA group）and 8 patients（control group,without CRA or any MetS factors）were collected.The expression of COX-2 in colonic mucosa was detected by immunohistochemistry（IHC）.COX-2 IHC staining scores and percentages of samples identified as COX-2 positive in epithelial layer,together with numbers of lamina propria COX-2 positive cells were assessed.The relationship between these 3 indexes and general clinical characteristics such as sex,age,smoking history,alcohol history,history of gallbladder removal,history of gastrointestinal neoplasms,and family history of colorectal neoplasms,were analyzed.CRA patients were divided into two groups based on whether they were with advanced adenomas or MetS factors,and two groups were compared with each other.Results No general clinical characteristics were significantly associated with COX-2 expression in colonic normal mucosa（P〉0.05）.Numbers of lamina propria COX-2 positive cells in patients with advanced adenomas were significantly higher than control group（P〈0.05）.All 3 indexes above-mentioned in CRA patients with MetS factors were significantly higher than those without any MetS factors（P〈0.01）.Conclusion COX-2 expression in colonic mucosa of patients with CRA is significantly associated with MetS factors.

关 键 词：代谢综合征 结直肠肿瘤 腺瘤 环氧酶-2 

分 类 号：R735.3[医药卫生—肿瘤]