术中组织印片细胞DNA倍体分析在甲状腺疾病中的诊断意义  

Clinical significance of DNA ploidy analysis of intraoperative thyroid tissue touch preparation

作　　者：张林重 任力[2] 李炜[2] 李德昌[2] 岳颖[2] 郑吉春[2] 

机构地区：[1]河北北方学院,河北张家口075000 [2]空军总医院病理科,北京100142

出　　处：《诊断病理学杂志》2016年第8期593-596,共4页Chinese Journal of Diagnostic Pathology

基　　金：首都卫生发展科研专项基金(首发2011-5041-01)

摘　　要：目的探讨术中组织印片细胞DNA倍体分析对甲状腺疾病良恶性的诊断价值,提高术中快速诊断的准确率。方法对549例甲状腺术中冷冻标本进行术中组织印片细胞DNA倍体分析及冷冻切片诊断,并与术后常规石蜡切片诊断、免疫组化及BRAF V600E基因突变检测结果进行比较。结果 549例甲状腺术中冷冻切片确诊536例,确诊率97.6%;术中组织印片细胞DNA倍体分析确诊543例,准确率98.9%;二者联合确诊545例,确诊率99.3%。结论将冷冻切片联合组织印片细胞DNA倍体分析应用于甲状腺疾病手术中快速病理检查,能减少误诊,提高快速诊断的准确率。Objective To investigate the diagnosis value of DNA ploidy analysis of intraoperative thyroid tissue touch preparation, and to improve the accuracy of the rapidly intraoperative diagnosis of the thyroid lesions. Methods 549 cases of the thyroid lesions were detected by touch preparation cytology and frozen section during operation, and the results were compared with the postoperative histopathologic diagnosis, immune phenotype and the status of BRAF V600E gene mutation. Results 536 cases were diagnosed properly with frozen section （536/549, 97.6% ） , 543 were diagnosed exactly with DNA ploidy analysis of intraoperative thyroid tissue on touch preparation cytology （543/549, 98.9% ） , and 545 cases were diagnosed properly by combination of touch preparation and frozen section （545/549, 99.3% ）. Conclusion Both DNA ploidy analysis of touch preparation cytology and frozen section applied during operation can increase the aceuracy of rapidly intraoperative diagnosis for the thyroid lesions.

关 键 词：甲状腺疾病 术中病理诊断 DNA倍体分析 

分 类 号：R736.1[医药卫生—肿瘤]