马齿苋多糖对溃疡性结肠炎小鼠肠黏膜sIgA及病理表现的影响  

Effects of polysaccharides from Portulaca oleracea L. on intestinal mucosal sIgA and pathological manifestations in mice with ulcerative colitis

作　　者：代月[1] 韩振忠 杨春佳[1] 王春敏[1] 任继秋[1] 吴庆田[1] 张昆[1] 崔刚[1] 武菲[1] 蔡连顺[1] 于莲[3] 马淑霞[1] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007 [2]黑龙江精准肿瘤康复医院检验科,黑龙江哈尔滨150000 [3]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《中国微生态学杂志》2016年第8期903-905,915,共4页Chinese Journal of Microecology

基　　金：黑龙江省教育厅课题(12531689);黑龙江省自然科学基金(H201363);国家自然科学基金(81274101);黑龙江省普通高等学校医学病原学(BSL3)重点实验室开放课题资助

摘　　要：目的探讨马齿苋多糖对溃疡性结肠炎小鼠肠黏膜sIgA及病理表现的影响。方法应用硫酸葡聚糖钠(DSS)制备溃疡性结肠炎小鼠模型,随机分成两组:正常对照组、模型组,造模成功后模型组再分为自然恢复组、马齿苋多糖治疗组。分别于造模后、给药7d后处死小鼠,进行肠道菌群、肠黏膜sIgA及结肠组织病理学检测。结果 DSS造模后模型组小鼠肠道菌群失调、肠黏膜sIgA含量下降、结肠组织有病理改变。马齿苋多糖治疗7d后治疗组小鼠肠道双歧杆菌和乳酸杆菌数量明显上升,肠黏膜sIgA含量上升、结肠组织病理改变减轻。结论马齿苋多糖可以提高双歧杆菌和乳酸杆菌数量,提高肠黏膜sIgA含量,改善结肠组织病理变化,对溃疡性结肠炎发挥了一定的治疗作用。Objective To discuss the effects of polysaccharides fromPortulaca oleracea L.on intestinal mucosal sIgA and pathological manifestations in mice with ulcerative colitis.Methods Mice were randomly divided into the control group or model group induced by DSS（DSS group）.The mice with ulcerative colitis（DSS group）were further subdivided into natural recovery group or treatment group.After modeling and 7days of therapy,the mice were respectively sacrificed to detect the intestinal flora,intestinal mucosal sIgA and colon endotoxin.Results The mice in model group developed intestinal flora imbalance,their sIgA levels declined,and colon tissue appeared pathological changes after the treatment of DSS.After 7days of therapy,the quantities of intestinal bifidobacteria and lactobacilli increased significantly,the sIgA content in intestinal mucosa increased either,and pathological changes of colon tissue were improved.Conclusion Polysaccharides of Portulaca oleracea L.can increase the quantities of bifidobacteria and lactobacilli,increase the sIgA content,and improve pathological changes of colon tissue,which can help with the treatment of ulcerative colitis.

关 键 词：马齿苋多糖 SIGA 病理表现 

分 类 号：R285.5[医药卫生—中药学]